Figure 2: Purification of concentrated WSF by gel filtration- FPLC with HiPerp sephacryl S-200 high resolution column (length 600 mm, i.d. 16 mm). Elusion was done with BPS (0.15 M, pH 7.4) at flow rate of 0.17 ml/min, 1 ml for each fraction
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Figure 2: Purification of concentrated WSF by gel filtration- FPLC with HiPerp sephacryl S-200 high resolution column (length 600 mm, i.d. 16 mm). Elusion was done with BPS (0.15 M, pH 7.4) at flow rate of 0.17 ml/min, 1 ml for each fraction
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