Advanced Biomedical Research

REVIEW ARTICLE
Year
: 2015  |  Volume : 4  |  Issue : 1  |  Page : 120-

Conventional, molecular methods and biomarkers molecules in detection of septicemia


Mohammad Reza Arabestani, Sahar Rastiany, Sima Kazemi, Seyed Masoud Mousavi 
 Brucellosis Research Center; Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

Correspondence Address:
Dr. Mohammad Reza Arabestani
Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan
Iran

Sepsis is a leading cause of morbidity and mortality in hospitalized patients worldwide and based on studies, 30-40% of all cases of severe sepsis and septic shock results from the blood stream infections (BSIs). Identifying of the disease, performing laboratory tests, and consequently treatment are factors that required for optimum management of BSIs. In addition, applying precise and immediate identification of the etiologic agent is a prerequisite for specific antibiotic therapy of pathogen and thereby decreasing mortality rates. The diagnosis of sepsis is difficult because clinical signs of sepsis often overlap with other noninfectious cases of systemic inflammation. BSIs are usually diagnosed by performing a series of techniques such as blood cultures, polymerase chain reaction-based methods, and biomarkers of sepsis. Extremely time-consuming even to take up to several days is a major limitation of conventional methods. In addition, yielding false-negative results due to fastidious and slow-growing microorganisms and also in case of antibiotic pretreated samples are other limitations. In comparison, molecular methods are capable of examining a blood sample obtained from suspicious patient with BSI and gave the all required information to prescribing antimicrobial therapy for detected bacterial or fungal infections immediately. Because of an emergency of sepsis, new methods are being developed. In this review, we discussed about the most important sepsis diagnostic methods and numbered the advantage and disadvantage of the methods in detail.


How to cite this article:
Arabestani MR, Rastiany S, Kazemi S, Mousavi SM. Conventional, molecular methods and biomarkers molecules in detection of septicemia.Adv Biomed Res 2015;4:120-120


How to cite this URL:
Arabestani MR, Rastiany S, Kazemi S, Mousavi SM. Conventional, molecular methods and biomarkers molecules in detection of septicemia. Adv Biomed Res [serial online] 2015 [cited 2020 Oct 25 ];4:120-120
Available from: https://www.advbiores.net/article.asp?issn=2277-9175;year=2015;volume=4;issue=1;spage=120;epage=120;aulast=Arabestani;type=0