Advanced Biomedical Research

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 3  |  Issue : 1  |  Page : 113-

Evaluation of repair in duodenal perforation with human amniotic membrane: An animal model (dog)


Leila Ghahramani, Ali Bagherpour Jahromi, Mohammad Reza Dehghani, Mohammad Javad Ashraf, Salar Rahimikazerooni, Abbas Rezaianzadeh, Ali Reza Safarpour, Seyed Vahid Hosseini 
 Colorectal Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

Correspondence Address:
Salar Rahimikazerooni
Colorectal Research Center, Shiraz University of Medical Sciences, Shiraz
Iran

Background: There is a growing tendency toward application of human amniotic membrane (HAM) as a biologic substitute in various tissue injuries where a significant tissue loss is a matter of concern. In gastrointestinal injuries especially duodenal ones, some potential limitations in current surgical techniques contribute to not fully acceptable healing outcomes. Thus, this study was carried out to assess repair with HAM patch for duodenal defect in comparison with simple duodenoraphy in an animal model (dog). Materials and Methods: A total of 15 male German shepherd dogs weighing 23-27 kg were randomly divided into two groups. Group A with 10 dogs, which were a candidate for duodenal repair by amniotic membrane patch and Group B consisted of 5 dogs perform simple duodenorraphy. A precise control was made to match all conditions except surgical technique. Macroscopic and microscopic features of the healed duodenal lumen in both groups were recorded. Results: Gross evaluation revealed no difference in luminal diameter in both groups. Statistical analysis of duodenal diameter between both groups after operation also showed no significant difference (Pv = 0.789). Histological assessment indicated less inflammation with better wound healing in Group A. Conclusion: It seems that repairing duodenal wall defect with HAM would result in better histological outcomes compared with what is seen in simple duodenoraphy in animal models. However, there is no significant difference regarding surgical findings.


How to cite this article:
Ghahramani L, Jahromi AB, Dehghani MR, Ashraf MJ, Rahimikazerooni S, Rezaianzadeh A, Safarpour AR, Hosseini SV. Evaluation of repair in duodenal perforation with human amniotic membrane: An animal model (dog).Adv Biomed Res 2014;3:113-113


How to cite this URL:
Ghahramani L, Jahromi AB, Dehghani MR, Ashraf MJ, Rahimikazerooni S, Rezaianzadeh A, Safarpour AR, Hosseini SV. Evaluation of repair in duodenal perforation with human amniotic membrane: An animal model (dog). Adv Biomed Res [serial online] 2014 [cited 2020 Dec 2 ];3:113-113
Available from: https://www.advbiores.net/article.asp?issn=2277-9175;year=2014;volume=3;issue=1;spage=113;epage=113;aulast=Ghahramani;type=0