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Original Article:
Optimization and Comparison of Different Methods and Factors for Efficient Transformation of
Brucella abortus
RB51strain
Azam Gheibi, Hossein Khanahmad, Gholam Ali Kardar, Maryam Boshtam, Sassan Rezaie, Bahram Kazemi, Mohammad Reza Khorramizadeh
Adv Biomed Res
2019, 8:37 (30 May 2019)
DOI
:10.4103/abr.abr_14_19
PMID
:31198771
Background:
The development of protective vaccines for
Brucella
spp. has been hampered by the difficulty in transformation of
Brucella
cells with foreign DNA for genetic manipulation. It seems that the formation of
Brucella
spheroplasts would increase the efficiency of transformation. The aim of this study was to devise an efficient method for the transformation of
Brucella
spp.
Materials and Methods:
At first, spheroplast of
Brucella
was prepared by glycine and ampicillin induction and transformed using optimized protocols of CaCl
2
, electroporation, and lipofection methods. Then, the efficacy of transformation was compared between the three-mentioned methods.
Results:
Ampicillin-induced spheroplasts from early-log phase culture of brucella when incubated in a medium-containing 0.2 M sucrose during cell recovery had higher transformation efficiency in three different methods. Comparison of the transformation efficiency of
Brucella
abortus RB51 using the CaCl2, lipofection, and electroporation methods revealed that the transformation efficiency with the lipofection method was significantly higher than with other two methods (P < 0.05).
Conclusions:
Lipofection method by lipofectamine 2000 on ampicillin-induced spheroplasts can be a suitable approach for
Brucella
transformation.
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Original Article:
Internal Urethrotomy in Treatment of Female with Anatomical Bladder Outlet Obstruction
Hourieh Sharifian, Mahtab Zargham, Mohamad Hatef Khorami, Mehrdad Mohamadi, Hamid Mazdak, Sara Mozafarpour
Adv Biomed Res
2019, 8:36 (27 May 2019)
DOI
:10.4103/abr.abr_200_18
PMID
:31259165
Background:
Bladder outlet obstruction (BOO) is a relatively infrequent urologic condition in women, but can cause bothersome symptoms. In this article, transurethral incisions of the bladder neck (TUIBN) and urethra in the treatment of anatomical BOO were assessed.
Materials and Methods:
A total of 23 women who referred with chronic lower urinary tract symptoms, urinary retention, and difficulty in micturition were assessed. Diagnose was made on the basis of urethrocystoscopy, voiding cystourethrography, and urodynamic studies. All patients underwent transurethral incisions of bladder neck contracture or site of observed urethral stenosis at 3- and 9-o'clock positions. Clinical improvements and complications were assessed by follow-up examination and International Prostate Symptom Score (IPSS) and quality of life (QOL) scoring before and after intervention.
Results:
All patients were followed for at least 6 months after intervention. Follow-up data were available for 19 (90.5%), 13 (61.9%), and 7 (33.3%) of patients at 12, 24, and 48 months' follow-up, respectively. During the follow-up period, the mean IPSS and QOL significantly changed from 26.84 to 10.74 and 4.76 to 2.32, respectively (
P
< 0.001). Approximately 66.7% had satisfactory outcomes, 47.7% patients after first, and 19.1% after second TUI. About9.5% patients developed new onset of stress urinary incontinence, one of them had indications of surgical intervention, and thus, symptoms were relieved by mid-urethral sling.
Conclusion:
Transurethral incision of bladder neck and urethra seems to be effective in relieving urinary symptoms of anatomical BOO in women. Complications may rarely occur and can be fully managed.
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Case Report:
A Case of Mayer–Rokitansky–Küster–Hauser Syndrome with a Fused Pancake-shaped Pelvic Kidney
Ali Reza Eftekhari Moghadam, Ghasem Saki, Mahin Taheri Moghadam, Seyed Mohamad Hossein Mohseni, Vajieh Heidari, Mohammad Hossein Jamshidi
Adv Biomed Res
2019, 8:35 (27 May 2019)
DOI
:10.4103/abr.abr_97_18
PMID
:31259164
Mayer–Rokitansky–Küster–Hauser (MRKH) Syndrome is a female reproductive system disorder. It is characterized by a defect in the Müllerian ducts development, and it causes the absence of the uterus in variable degrees in upper vaginal hypoplasia. In addition, it is often associated with the unilateral renal dysplasia. Müllerian agenesis affects 1 in 4500 newborn girls and is considered as a sporadic anomaly. Women with MRKH Syndrome have a normal female chromosome pattern 46, XX with normal ovarian function. The presence of bilateral kidney agenesis with a pelvic pancake-shaped kidney is a rare condition, and a few cases have been reported in medical journals. This case study focuses on a case of MRKH Syndrome with bilateral renal agenesis and a pancake-shaped kidney.
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Original Article:
Trans-scrotal Incision Approach versus Traditional Trans-scrotal Incision Orchiopexy in Children with Cryptorchidism: A Randomized Trial Study
Masoud Nazem, Mehrdad Hosseinpour, Abdulhakim Alghazali
Adv Biomed Res
2019, 8:34 (27 May 2019)
DOI
:10.4103/abr.abr_26_19
PMID
:31259163
Background:
Although undescended testis (UDT) is the most prevalent malformation in male neonates, the best mode of UDT treatment remains controversial. This study aimed to compare trans-scrotal incision approach with traditional trans-scrotal incision orchiopexy in children suffering from cryptorchidism.
Materials and Methods:
This single-blind randomized clinical trial was done on 100 children with UDT who needed surgery. The participants were alternately undergoing trans-scrotal incision orchiopexy (Group I) and traditional inguinal incision orchiopexy (Group II). The success rate and incidence of postoperative complications were evaluated 1 week and 1 month and 6 months after the operation in the two groups.
Results:
Both the groups were similar in baseline characteristics including age and laterality (
P
> 0.05). There was no significant difference between the two groups in terms of the incidence of wound infection, testicular atrophy, testicular hypotrophy, and relapse (
P
> 0.05). In addition, the success rates were 98% in Group I and 94% in Group II (
P
> 0.05).
Conclusion:
Both surgical methods have a high success rate, and there is no significant difference in the incidence of complications; however, in terms of beauty, satisfaction, and shortening the duration of surgery and the duration of hospitalization, trans-scrotal approach was more successful than the traditional method.
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Original Article:
Targeting MCF-7 Cell Line by Listeriolysin O Pore Forming Toxin Fusion with AHNP Targeted Peptide
Gholamreza Fotoohi-Ardakani, Majid Kheirollahi, Hossein Zarei Jaliani, Mohadese Noorian, Hossein Ansariniyia
Adv Biomed Res
2019, 8:33 (27 May 2019)
DOI
:10.4103/abr.abr_18_19
PMID
:31259162
Background:
Tumor-targeting peptides are attracting subjects in cancer therapy. These peptides, which are widely studied, deliver therapeutic agents to the specific sites of tumors. In this study, we produced a new form of recombinant listeriolysin O (LLO) with genetically fused Anti-HER2/neu peptide (AHNP) sequence adding to its C-terminal end. The aim of the study was to engineer this pore-forming toxin to make it much more specific to tumor cells.
Materials and Method and Results:
Two forms of the toxin (with and without peptide) were subcloned into a bacterial expression plasmid. Subcloning was performed using a polymerase chain reaction (PCR) product as a megaprimer in a quick-change PCR to introduce the whole insert gene into the expression plasmid. After expression of two recombinant forms of LLO in BL21 DE3 cells, purification was performed using Ni-NTA affinity column. MDA-MB-231 and MCF-7 cell lines (as negative and positive controls, respectively) were treated with both LLO toxins to evaluate their cytotoxicity and specificity. The IC
50
of LLO on MDA-MB-231 and MCF-7 cells was 21 and 5 ng/ml, respectively. In addition, IC
50
for the fusion AHNP-LLO toxin was 140 and 60 ng/ml, respectively. It was found that the cytotoxicity of the new engineered AHNP-LLO toxin has decreased by about 9x compared to the wild-type toxin and the specificity of the AHNP-LLO toxin has been also reduced.
Conclusions:
Results show that the C-terminal of the LLO should not be modified and it seems that N-terminal of the toxin should be preferred for engineering and adding peptide modules.
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2022
April
[
9
]
March
[
8
]
February
[
9
]
January
[
10
]
2021
December
[
10
]
November
[
11
]
October
[
5
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September
[
6
]
August
[
2
]
July
[
4
]
June
[
3
]
May
[
1
]
March
[
2
]
February
[
4
]
January
[
6
]
2020
December
[
8
]
November
[
7
]
October
[
13
]
September
[
10
]
August
[
10
]
July
[
7
]
June
[
4
]
May
[
5
]
April
[
5
]
March
[
3
]
February
[
4
]
January
[
5
]
2019
December
[
2
]
November
[
5
]
October
[
5
]
September
[
7
]
August
[
6
]
July
[
3
]
June
[
7
]
May
[
5
]
April
[
8
]
March
[
6
]
February
[
8
]
January
[
10
]
2018
December
[
9
]
November
[
9
]
October
[
11
]
September
[
5
]
August
[
10
]
July
[
10
]
June
[
13
]
May
[
17
]
April
[
17
]
March
[
19
]
February
[
19
]
January
[
20
]
2017
December
[
13
]
November
[
21
]
October
[
12
]
September
[
9
]
August
[
15
]
July
[
22
]
June
[
11
]
May
[
14
]
April
[
14
]
March
[
21
]
February
[
8
]
January
[
8
]
2016
December
[
17
]
November
[
20
]
October
[
12
]
September
[
9
]
August
[
19
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July
[
16
]
June
[
17
]
May
[
17
]
April
[
16
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March
[
36
]
February
[
14
]
January
[
14
]
2015
December
[
2
]
November
[
15
]
October
[
25
]
September
[
29
]
August
[
29
]
July
[
31
]
June
[
11
]
May
[
39
]
March
[
15
]
February
[
29
]
January
[
34
]
2014
December
[
23
]
November
[
25
]
October
[
18
]
September
[
19
]
August
[
22
]
July
[
13
]
June
[
11
]
May
[
22
]
April
[
2
]
March
[
24
]
February
[
11
]
January
[
77
]
2013
November
[
10
]
October
[
5
]
August
[
1
]
July
[
23
]
June
[
11
]
March
[
42
]
2012
December
[
4
]
October
[
17
]
August
[
34
]
July
[
15
]
May
[
10
]
March
[
7
]
1900
January
[
1
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Online since 15 January, 2012