Users Online: 313
Home
About us
Editorial board
Search
Browse articles
Submit article
Ahead of Print
Instructions
Subscribe
Contacts
Login
» Articles published in the past year
To view other articles click corresponding year from the navigation links on the side bar.
All
|
Brief Communication
|
Brief Reports
|
Case Report and Literature Review
|
Case Reports
|
Editorial
|
Erratum
|
Letter to Editor
|
Letters to Editor
|
Notice of Retraction
|
Original Article
|
Original Articles
|
Research Articles
|
Review Articles
|
Review Report
|
Short Communications
|
Systematic Review
Export selected to
Endnote
Reference Manager
Procite
Medlars Format
RefWorks Format
BibTex Format
Show all abstracts
Show selected abstracts
Export selected to
Add to my list
Research Article:
Effect of teicoplanin on the expression of c-myc and c-fos proto-oncogenes in MCF-7 breast cancer cell line
Saeideh Ashouri, Maryam Hosseindokht Khujin, Mohammad Kazemi, Majid Kheirollahi
Adv Biomed Res
2016, 5:172 (28 November 2016)
DOI
:10.4103/2277-9175.190984
PMID
:28028512
Background:
Teicoplanin is a member of vancomycin-ristocetin family of glycopeptide antibiotics. It mediated wound healing by increasing neovascularization possibly through activation of MAP kinase signaling pathway. The aim of this study is an evaluation of c-myc and c-fos genes expression after treatment of cells by teicoplanin and determines whether this glycopeptide antibiotic exerts its proliferation effects by influencing the expression of these genes. Hence, this study was designed to elucidate one possible mechanism underlying teicoplanin effects on cell proliferation using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay.
Materials and Methods:
Breast cancer cell line, MCF-7, was cultured, and three different concentrations of teicoplanin were added to the plates. We measured the cell proliferation rate by MTT assay. After cell harvesting, total RNA was extracted to synthesize single-stranded cDNA. Real-time polymerase chain reaction was performed, and the data were analyzed.
Results:
It was observed that the level of c-fos and c-myc genes' expressions was decreased at all three different concentrations of teicoplanin.
Conclusion:
it could be concluded that although teicoplanin is considered as an enhancing cell growth and proliferation, but probably its effect is not through MAP kinase signaling pathway or perhaps even has inhibitory effect on the expression of some genes such as c-myc and c-fos in this pathway. Hence, the mechanism of action of teicoplanin for increasing cell propagation, through cell signaling pathways or chromosomal abnormalities, remains unclear, and further studies should be conducted.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (2) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Research Article:
Adverse effects of BCG vaccine 1173 P2 in Iran: A meta-analysis
Saied Mostaan, Bahador Yazdanpanah, Rasool Moukhah, Hamid Reza Hozouri, Manouchehr Rostami, Mohsen Khorashadizadeh, Javad Zerehsaz, Ramin Pirhajati Mahabadi, Arya Saadi, Hossein Khanahmad, Mohammad Pooya
Adv Biomed Res
2016, 5:99 (8 June 2016)
DOI
:10.4103/2277-9175.183659
PMID
:27376038
Although in the last two decades the World Health Organization (WHO) has introduced tuberculosis as “a threat to global”, the vaccination with the
Mycobacterium bovis
Bacillus Calmette-Guerin (BCG) is the only way for the prevention of this fatal infectious disease. Despite of the efficacy of BCG vaccine especially against infants' meningitis, it has still some limitations due to a variety of adverse effects. Many studies have evaluated the side effects of different strains of BCG vaccines in different countries. In Iran, some studies have been done so far to evaluate the adverse effects of 1173 P2 strain which is used for BCG vaccination. Each of these studies have used different standardization and sampling methods. This review will survey all studies that have been published about adverse effects of 1173 P2 strain of BCG vaccine in Iran using data mining methods.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (2) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Feedback
Subscribe
Advanced Search
Month wise articles
Figures next to the month indicate the number of articles in that month
2022
February
[
1
]
2021
November
[
1
]
2018
March
[
1
]
2017
November
[
1
]
2016
November
[
1
]
June
[
1
]
2015
September
[
1
]
July
[
1
]
2013
August
[
1
]
2012
July
[
1
]
Sitemap
|
What's New
Feedback
|
Copyright and Disclaimer
|
Privacy Notice
© Advanced Biomedical Research | Published by Wolters Kluwer -
Medknow
Online since 15 January, 2012