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Adv Biomed Res 2020,  9:25

Molecular genetic study in a cohort of Iranian families suspected to maturity-onset diabetes of the young, reveals a recurrent mutation and a high-risk variant in the CEL gene

1 Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
2 Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Isfahan Endocrine and Metabolism Research Center, Isfahan University of Medical Science, Isfahan, Iran
4 Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
5 Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences; Pediatric Inherited Diseases Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran

Date of Submission21-Jan-2020
Date of Decision01-Mar-2020
Date of Acceptance07-Apr-2020
Date of Web Publication27-Jun-2020

Correspondence Address:
Dr. Mohammad Amin Tabatabaiefar
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Hezarjarib St., Isfahan
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/abr.abr_18_20

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Background: Diabetes mellitus (DM) is a group of metabolic disorders in the body, accompanied with increasing blood sugar levels. Diabetes is classified into three groups: Type 1 DM (T1DM), Type 2 DM (T2DM), and monogenic diabetes. Maturity-onset diabetes of the young (MODY) is a monogenic diabetes that is frequently mistaken for T1D or T2D. The aim of this study was to diagnose MODY and its subtype frequency in a diabetic population in Iran. Materials and Methods: In this study among ten diabetic families that were highly suspected to MODY by nongenetic biomarkers and without any pathogenic mutation in GCK and HNF1A genes, two patients from two unrelated families were examined via whole-exome sequencing (WES) in order to detect the causative gene of diabetes. Co-segregation analysis of the identified variant was performed using Sanger sequencing. Results: In this study, no pathogenic variant was found in GCK and HNF1A genes (MODY2 and MODY3), while these two types of MODY were introduced as the most frequent in other studies. By using WES, a pathogenic variant (p.I488T) was found in one of the patients in CEL gene causing MODY8 that its frequency is very rare in other studied populations. A high-risk variant associated with diabetes was found in another patient. Conclusion: WES was applied in this study to reveal the cause of MODY in 1 family. This pathogenic mutation was previously reported as a disease causing mutation.

Keywords: Carboxyl ester lipase, maturity-onset diabetes of the young, pathogenic variant, whole-exome sequencing

How to cite this article:
Sarmadi A, Mohammadi A, Tabatabaei F, Nouri Z, Chaleshtori MH, Tabatabaiefar MA. Molecular genetic study in a cohort of Iranian families suspected to maturity-onset diabetes of the young, reveals a recurrent mutation and a high-risk variant in the CEL gene. Adv Biomed Res 2020;9:25

How to cite this URL:
Sarmadi A, Mohammadi A, Tabatabaei F, Nouri Z, Chaleshtori MH, Tabatabaiefar MA. Molecular genetic study in a cohort of Iranian families suspected to maturity-onset diabetes of the young, reveals a recurrent mutation and a high-risk variant in the CEL gene. Adv Biomed Res [serial online] 2020 [cited 2021 Apr 21];9:25. Available from:

  Introduction Top

Diabetes mellitus (DM) is a heterogeneous group of metabolic disorders, characterized by high levels of blood glucose.[1] Usually, diabetes occurs as a result of β-cell dysfunction and/or deficiency of insulin secretion or its action.[2] There are two common types of diabetes: Type 1 DM (T1DM) and type 2 DM (T2DM). T1DM is an autoimmune disorder, and increased levels of anti-pancreatic β-cells autoantibodies are seen in this type of diabetes. T1DM patients must receive exogenous insulin in an appropriate dose daily.[3] In T2DM, partial insulin deficiency is the notable feature of the disease, and it is due to impaired insulin secretion or insulin resistance.[4]

Monogenic types of diabetes account for about 2%–5% of all types of diabetes. Usually, mutations in genes related to β-cell functions can lead to monogenic types of diabetes. Maturity-onset diabetes of the young (MODY) and neonatal diabetes are monogenic types of diabetes. Syndromic diabetes are also classifies in monogenic diabetes categories.[5] One of the most common concerns of health-care providers is misdiagnosis of MODY types of diabetes with common types of diabetes (T1D and T2D), especially HNF1A-MODY is usually misclassified as T1D.[6] Therefore, improper management of blood sugar control may be adopted. In some subtypes of MODY such as MODY1 (HNF4A mutations) and MODY3 (HNF1A mutations), proper management of hyperglycemia is so important to avoid micro/macro vascular complications of eyes, kidney, and heart. Whereas, in some of the MODY subtypes such as MODY2 (GCK mutations), the blood sugar levels are slightly higher than normal, and so that, simple physical activity can prevent the outcomes of hyperglycemia.[7]

MODY accounts for 2%–5% of all types of diabetes. For the first time in 1974, Tattersall reported a “mild” type of diabetes in three families from King's College Hospital in London.[8] He discovered that it is a mild form of diabetes because many patients do not need necessarily insulin therapy and they had milder diabetes complications than expected.[9] Albeit Tattersall et al. recognized that these patients “have a type of diabetes that it is onset in young and it is different from T1D and T2D,” they did not use the term “maturity-onset diabetes of the young” for these types of diabetes.[10] MODY has several specific features that is use to distinguish it from other types of diabetes: Early onset of diabetes (<25 years), early onset diabetes in at least 2 or ideally three family generation, autosomal dominant mode of inheritance, noninsulin dependence (not requiring insulin even after 3 years of diagnosis) in most of its types, and nonketotic forms of diabetes.[11]

Mutations in at least 14 different genes are known to cause MODY.[12] The carboxyl ester lipase protein (CEL) is a pancreatic enzyme hydrolyzing cholesteryl esters and other dietary lipids. CEL or BSSL (MIM:114840) is located on human chromosome 9 (9q34.13), containing 11 exons and encodes a protein that is a glycoprotein secreted from the pancreas into the digestive tract.[13] Mutations in CEL have been associated with MODY8 and chronic pancreatitis.[14]CEL is expressed in the acinar cells of the exocrine pancreas and along with a variety of other lipases, released into the gut to break down complex lipids for absorption.[15] Recent evidence shows that mutations in the CEL gene can cause protein aggregation in the pancreas, which could induce tissue damage and lead to the symptoms of pancreatic dysfunction; it most likely results in MODY8.[16]

Despite its low prevalence, efforts in diagnosing and classification of MODY patients is important because it may clarify the understanding of genetic susceptibility in families predisposed to high risk of the disease. According to autosomal dominant mode of inheritance, every patient has 50% chance to have an affected child.[17]

The purpose of this study was to identify and diagnose patients with MODY among Isfahan province diabetic patients and determining the frequency of different types of MODY based on the mutated gene.

  Materials and Methods Top


The medical files of about 2000 diabetic patients with early-onset diabetes were checked to find MODY patients. Of them, about sixty cases having MODY criteria (onset age under 25, no insulin consumes or low dose usage, strong history of diabetes in the family, and no history for keto-acid attacks) were selected for more evaluations. Written informed consent and informational questionnaires were taken from all the individuals. Five milliliters of peripheral blood were drawn from affected and healthy members of selected families, and conserved in ethylenediaminetetraacetic acid-containing tubes.

Clinical examination

There are some nongenetic biomarkers that are important in distinguishing MODY from T1D and T2D such as anti-pancreatic beta cell autoantibodies and serum C-peptide levels.[17],[18] A few case reports described antibody-positive MODY patients with a prevalence of <1%.[19] Thus, we measured anti-insulin II, anti-GAD65 autoantibodies, and serum C-peptide levels in all the patients. Antibody positivity and undetectable levels of C-peptide were used as exclusion criteria for MODY in the present study. Finally, 35 families were selected for the study.

Molecular analysis

DNA extraction

DNA was extracted from peripheral blood lymphocytes using Cinnagen DNA extraction kit (E × 6071, Cinnaclon Company, Iran). A NanoDrop 2000 spectrophotometer (Thermo Scientific Inc., Wilmington, DE, USA) was used to determine DNA concentration and purity.

Polymerase chain reaction-sequencing and mutation screening of GCK and HNF1A genes

Based on previous studies in different populations, mutations in GCK (MODY2) and HNF1A (MODY3) genes account for about 30%–80% of MODY cases.[20],[21] Primers for all the 11 exons of the GCK gene and 10 exons of the HNF1A gene, encompassing at least 60 flanking nucleotides, were designed by primer3 ver. 0.4.0 ( All the exons of these two genes were amplified by polymerase chain reaction (PCR). DNA sequencing of the PCR products were carried out by an ABI 3730XL automated sequencer (Applied Biosystems, Macrogen, South Korea) using the same forward primers. Reaction conditions to amplify exons in 30 μl was as follows: 0.5 μl of each of the primers (10 μM), 4 μl DNA sample, 15 μl Ampliqon PCR master mix (Taq 2x Master Mix RED. 1.5 mM MgCl2, ID: 5200300-1250) and 10 μl ddH2O. All the variants in these two genes were analyzed.

Whole-exome sequencing

About 300 ng of genomic DNA was sent to Macrogen (South Korea) and was subjected to whole-exome sequencing (WES) using a NovaSeq 4000 platform (Illumina, US). The mean depth of coverage was × 100, and 92% of targeted regions were covered. After performing WES, the dataset was analyzed. The released raw data were converted to the FASTQ file. Bioinformatics analysis included using burrows-wheeler aligner for read mapping to the reference genome (hg19, NCBI Build 38), Picard for removal of duplicate reads, and GATK for variant calling. Variant filtering was performed based on MAF <1% (MAF: Minor Allele Frequency) in dbSNP version 147, 1000 genomes project phase 3 database, NHLBI GO exome sequencing project, exome aggregation consortium (ExAC), and Iranome (local database) for missense, nonsense, splice site, stop loss, start codon change, frame-shift, and in-frame indels. Then, all the variants were analyzed and in order to validate any of them, MutationTaster2, MutPred, SIFT, Mutation Assessor, PolyPhen-2, and PROVEAN were applied. The highest probable predictions were filtered. Then, the pathogenicity of these variants was evaluated by ACMG guideline.[22] The effect of variants on protein stability was assessed using mCSM.[23] Co-segregation analysis was done using forward and reverse primers for 10 that cover the variant region. The sequence of used primers was F: GGTTCTCAGCTTTCCCTTCC and R: GGGGCTCTGCCAGTAACTCT.

  Results Top

Clinical findings

Many studies show that most of the MODY patients are misdiagnosed as T1D patients. Patients with detectable C-peptide levels and no high levels of pancreatic autoantibodies are suspected to be MODY (with a higher probability than 95%). From a population of about 2000 diabetic patients with a duration of 3–7 years of diabetes and onset of disease before 25 years old, 35 families were suspected to MODY by clinical check out (pancreatic antibodies and C-peptide)

  Gckand Hnf1a Genes Sequencing Results Top

Among ten families in whom all the exons of GCK and HNF1A genes were sequenced in all their members (five for GCK and five for HNF1A), several nonpathogenic polymorphisms were found. dbSNP (, clinvar (, ExAC (, and 1000 genome databases were searched for detected variants, and any of them was not disease causing mutation.

  Whole-Exome Sequencing Results Top

After WES analysis in two patients, a missense mutation (p. Ilu488Thr) in the exon 10 of the CEL gene was detected in one of them [Table 1]. Mutations in the CEL gene can cause MODY8.[14] The proband (Isf-12) was a 24-year-old girl with a diabetes duration of 6 years. She was from a consanguineous family ( first cousins) with a strong history of diabetes in the family [pedigree is shown in [Figure 1]. While the proband and her older diabetic brother were treated with oral hypoglycemic drugs at first, both of them and also their father, were receiving insulin for about 4–6 years after the onset of the disease. The variant (p. Ilu488Thr) was previously reported as a pathogenic variant in 2014.[24] The frequency of this variant is 0.01 in Iranian population ( [Table 2].
Table 1: In silico analysis of identified variants in the carboxyl ester lipase gene

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Figure 1: (i) The pedigree of family Isf-12. Co-segregation of the variant in the members of the family is shown by +: being positive for the variant and −: being negative for the variant. (ii) The electropherogram of the muatation co-segregation in the family. (a) The proband with c.1463T>C heterozygously, (b) her diabetic father with the same variant heterozygously, and (c) her healthy mother with T nucleotide in that position homozygously

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Table 2: Population frequency of p.I488T in carboxyl ester lipase gene in different populations of Iran

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Co-segregation analysis showed variant segregation with diabetes in heterozygous state in all affected members of the family. The electropherogram of the variant region in the proband, her diabetic father, and her healthy mother is shown in [Figure 1] and [Figure 2].
Figure 2: The pedigree of family Isf-9. The proband in this study was number 14

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The second proband was a 16-year-old girl suffering from diabetes for 4 years. Her father, grandmother, 2 uncles, aunt, and 1 cousin were diabetic too [pedigree is shown in [Figure 2]. Metformin was prescribed for glycemic control as a choice drug. The affected father suffering from hyperglycemia, hypercholesterolemia, and high blood pressure is receiving insulin now, after treating with glibenclamide for 5 years. WES detected a missense variant in the exon 9 of CEL gene. The variant was named as p. Thr412Ilu by MutationTaster and its Reference ID is Rs62576769. It was not found in the 1000 Genome project, but it has a frequency of 5599 in the ExAC database in heterozygous state (0 allele homozygous state). The prediction was disease causing in this online software because it is situated in a highly conserved region of the protein. SIFT and Polyphen scores described it as a deleterious variant [Table 1]. Co-segregation analysis of this variant was also done and confirmed that it only presents in the diabetic members in heterozygous state ant it was absent in the healthy members of the family. Although there were lots of supporting evidence to confirm its pathogenicity, its MAF score and the high prevalence in the Iranian population [>0.05; [Table 3] is a strong reason for its rejection as a pathogenic mutation (
Table 3: Population frequency of p.T412I in carboxyl ester lipase gene in different populations of Iran

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  Discussion Top

MODY accounts for at least 2%–5% of all diabetes cases.[25] To date, heterozygous mutations in 14 genes have been reported for MODY subtypes [Table 4].[12] Early onset of diabetes (before the age of 25), strong family history for diabetes, autosomal dominant pattern of inheritance, and noninsulin dependent diabetes are the most important features of MODY.[11] Considering that most of MODY cases are misdiagnosed as T1D or T2D, and due to the importance of diagnosing this type of diabetes, the aim of this study was to identify and determine the subtypes of MODY in a cohort of diabetic patients in Isfahan province of Iran.
Table 4: Molecular and clinical characteristics of maturity-onset diabetes of the young subtypes

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To date, 14 different types of MODY have been reported until now. MODY8 (OMIM 609812, CEL-MODY) is the result of heterozygous mutations in the CEL gene, which accounts for <1% of all MODY cases.[27] This gene is located on chromosome 9q34.13 and consists of 11 exons. The CEL gene is not transcribed in beta cells, but is mostly expressed in pancreatic acinar cells[28],[29] and lactating mammary glands.[30] CEL is a CEL product that is secreted from the pancreas as a glycoprotein into the digestive tract, and it hydrolyzes dietary fat, cholesteryl esters, and fat-soluble vitamins in the duodenum.[31] Symptoms of CEL mutations/MODY8 are pancreatic lipomatosis (fatty replacement of pancreatic parenchyma) and fecal elastase levels manifesting before the age of 25, followed by the development of diabetes and pancreatic cysts later in life and lipomatosis.[14] The CEL protein represents 4%–8% of the total secreted protein in human pancreatic juice.[32] The mutant protein forms intracellular and extracellular aggregates, and misfolding of the protein leads to MODY8.[33]

Pathogenic gain-of-function effect of the altered protein has been shown as the cause of the diabetes by activation of maladaptive cell signaling pathways.[34],[35] Vesterhus et al. in 2010 showed that mice with knocked-out CEL gene do not show diabetes or exocrine dysfunction, indicating that MODY in CEL mutated cases is not due to haploinsufficiency.[36] In another study, patients with MODY8 indicated that the mutant CEL protein causes inflammatory changes in pancreatic tissue, expressing a cytotoxic link between mutant CEL and cellular proteins.[37]

The most common type of mutation that causes MODY in this gene is missense/nonsense (6 of 13 described in HGMD-professional 2019.1) ( Six of these 13 mutations cause MODY and/hypercholesterolemia, three cause only MODY, two cause diabetes and pancreatic exocrine dysfunction, and the other two cause high level of low-density lipoprotein (LDL)-cholesterol and chronic pancreatitis. These data and the clinical examination of our first proband and his family were clues that the variant is pathogenic. The father, the grandmother, and the affected uncles of the proband had high levels of LDL, but the proband (25 years old girl) and her 31-year-old brother, have not shown evidence of high LDL levels yet.

The most common types of MODY were reported as MODY2 and MODY3 in many studies in populations other than Iran.[38] However, there are few studies on MODY to provide data concerning the frequency of different types of MODY in Iran. Moghbeli et al., in 2017 reported that among 34 Iranian families of MODY patients, only 2% of them harbored mutations in HNF1A gene.[39] Whereas, in our previous study in 2019, we reported a frequency of 20% for HNF1A gene mutation from a population of ten patients with MODY criteria in Iran.[40] Thus, it seems that the frequency of different types of MODY in Iran is different to other populations and more studies on larger populations are needed. Similarly, in a study in 2015 in China, the authors showed that the frequency of mutated genes among Asian and other populations was different.[41] In our study, no pathogenic variant in GCK and HNF1A genes was found. However, using Sanger sequencing of all exons of these corresponding genes, several single-nucleotide polymorphisms (SNPs) were identified which have been associated with T2D. For example, George et al., in 2014 showed that the T nucleotide in the position of rs2908274 in exon 6 of the GCK gene has association with MODY2.[42] Another variant was rs1169310. The A allele is associated with increased plasma hsCRP levels, MODY, and T2D.[43] In a study in India using WES to indicate the cause of disease in 56 MODY patients, in addition to 12 pathogenic mutations they have reported, they also found some potentially damaging variants related to diabetes in HNF4A, GCK, HNF1A, PDX1, HNF1B, NEUROD1, and PAX4. However, they did not meet the criteria for being categorized as pathogenic variants. This could demonstrate a much more complex landscape of MODY.[44] The p.T412I polymorphism which was found in our second proband was found to be co-segregating in all members of the family, which was reported in another study in 2015 in China.[41] Therefore, it might be a high-risk variant contributing to diabetes. In agreement with the findings, we found that this variant fulfilled several lines of evidence, suggesting it as a high-risk variant: (1) This variant was introduced as a pathogenic variant abased on its SIFT, PolyPhen, REVEL, and PANTHER score and Mutation Taster prediction [Table 1] and (2) ΔΔG of this variant was assessed using a programme mCSM and it showed that the variant might result in destabilization of the protein (ΔΔG = −0.453).[23]

However, due to the high frequency of this variant, it could not be considered a pathogenic variant in our population. Therefore, our study supports the evidence that this variant is a high-risk factor for developing familial diabetes.

As in many studies on different populations, a high percentage of MODY patients did not show mutations on known genes, these so-called MODYX types should be subjected to further studies for possible identification of new genes and gene interactions.

  Conclusion Top

Few studies have been performed on MODY and its subtypes in the Iranian population. Our results suggest that mutations in the CEL gene might be involved in early-onset diabetes. The gene should be studied in a larger sample size in Iran for a more thorough understanding of its role in causing MODY. In this study, WES was employed to determine the type of MODY in one Iranian family. In the second family, another variant (p.T412I) was identified. Based on our current understanding, it does not meet the pathogenic criteria. However, it could well be a high-risk susceptibility variant. Further studies using WES are warranted to clarify the mutation profile of MODY in Iran.


We sincerely thank all patients and their families. The study was supported by Shahrekord University of Medical Sciences (grant no. 2596-70-01-1394), Isfahan University of Medical Science (grant no. 395116), and Isfahan Endocrine and Metabolism Research Center.

Financial support and sponsorship

The study was supported by Shahrekord University of Medical Sciences (grant no. 2596-70-01-1394), Isfahan University of Medical Science (grant no. 395116), and Isfahan Endocrine and Metabolism Research Center.

Conflicts of interest

There are no conflict of interest.

  References Top

American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care 2014;37 Suppl 1:S81-90.  Back to cited text no. 1
Bansal V, Gassenhuber J, Phillips T, Oliveira G, Harbaugh R, Villarasa N, et al. Spectrum of mutations in monogenic diabetes genes identified from high-throughput DNA sequencing of 6888 individuals. BMC Med 2017;15:213.  Back to cited text no. 2
Katsarou A, Gudbjörnsdottir S, Rawshani A, Dabelea D, Bonifacio E, Anderson BJ, et al. Type 1 diabetes mellitus. Nat Rev Dis Primers 2017;3:17016.  Back to cited text no. 3
DeFronzo RA, Ferrannini E, Groop L, Henry RR, Herman WH, Holst JJ, et al. Type 2 diabetes mellitus. Nat Rev Dis Primers 2015;1:15019.  Back to cited text no. 4
Greeley SA, Naylor RN, Philipson LH, Bell GI. Neonatal diabetes: An expanding list of genes allows for improved diagnosis and treatment. Curr Diab Rep 2011;11:519-32.  Back to cited text no. 5
Delvecchio M, Mozzillo E, Salzano G, Iafusco D, Frontino G, Patera PI, et al. Monogenic Diabetes Accounts for 6.3% of Cases Referred to 15 Italian Pediatric Diabetes Centers During 2007 to 2012. J Clin Endocrinol Metab 2017;102:1826-34.  Back to cited text no. 6
Anık A, Çatlı G, Abacı A, Böber E. Maturity-onset diabetes of the young (MODY): An update. J Pediatr Endocrinol Metab 2015;28:251-63.  Back to cited text no. 7
Tattersall R. Maturity-onset diabetes of the young: A clinical history. Diabet Med 1998;15:11-4.  Back to cited text no. 8
Tattersall RB, Fajans SS. A difference between the inheritance of classical juvenile-onset and maturity-onset type diabetes of young people. Diabetes 1975;24:44-53.  Back to cited text no. 9
Tattersall RB. Mild familial diabetes with dominant inheritance. Q J Med 1974;43:339-57.  Back to cited text no. 10
Siddiqui K, Musambil M, Nazir N. Maturity onset diabetes of the young (MODY)--history, first case reports and recent advances. Gene 2015;555:66-71.  Back to cited text no. 11
Radha V, Mohan V. Genetic basis of monogenic diabetes. Curr Sci 2017;113:1277.  Back to cited text no. 12
Torsvik J, Johansson S, Johansen A, Ek J, Minton J, Raeder H, et al. Mutations in the VNTR of the carboxyl-ester lipase gene (CEL) are a rare cause of monogenic diabetes. Hum Genet 2010;127:55-64.  Back to cited text no. 13
Kolar MJ, Kamat SS, Parsons WH, Homan EA, Maher T, Peroni OD, et al. Branched Fatty Acid Esters of Hydroxy Fatty Acids Are Preferred Substrates of the MODY8 Protein Carboxyl Ester Lipase. Biochemistry 2016;55:4636-41.  Back to cited text no. 14
Aho HJ, Sternby B, Kallajoki M, Nevalainen TJ. Carboxyl ester lipase in human tissues and in acute pancreatitis. Int J Pancreatol 1989;5:123-34.  Back to cited text no. 15
Torsvik J, Johansson BB, Dalva M, Marie M, Fjeld K, Johansson S, et al. Endocytosis of secreted carboxyl ester lipase in a syndrome of diabetes and pancreatic exocrine dysfunction. J Biol Chem 2014;289:29097-111.  Back to cited text no. 16
Kleinberger JW, Pollin TI. Undiagnosed MODY: Time for Action. Curr Diab Rep 2015;15:110.  Back to cited text no. 17
Sanyal D, Majumder A, Chaudhuri SR, Chatterjee S. Thyroid profile and autoantibodies in Type 1 diabetes subjects: A perspective from Eastern India. Indian J Endocrinol Metabolism 2017;21:45.  Back to cited text no. 18
McDonald TJ, Colclough K, Brown R, Shields B, Shepherd M, Bingley P, et al. Islet autoantibodies can discriminate maturity-onset diabetes of the young (MODY) from Type 1 diabetes. Diabet Med 2011;28:1028-33.  Back to cited text no. 19
Amed S, Oram R. Maturity-Onset Diabetes of the Young (MODY): Making the Right Diagnosis to Optimize Treatment. Can J Diabetes 2016;40:449-54.  Back to cited text no. 20
Irgens HU, Molnes J, Johansson BB, Ringdal M, Skrivarhaug T, Undlien DE, et al. Prevalence of monogenic diabetes in the population-based Norwegian Childhood Diabetes Registry. Diabetologia 2013;56:1512-9.  Back to cited text no. 21
Richards S, Aziz N, Bale S, Bick D, Das S, Gastier-Foster J, et al. Standards and guidelines for the interpretation of sequence variants: A joint consensus recommendation of the American College of Medical Genetics and Genomics and the Association for Molecular Pathology. Genet Med 2015;17:405-24.  Back to cited text no. 22
Pires DE, Ascher DB, Blundell TL. mCSM: Predicting the effects of mutations in proteins using graph-based signatures. Bioinformatics 2014;30:335-42.  Back to cited text no. 23
Johansen CT, Dubé JB, Loyzer MN, MacDonald A, Carter DE, McIntyre AD, et al. LipidSeq: A next-generation clinical resequencing panel for monogenic dyslipidemias. J Lipid Res 2014;55:765-72.  Back to cited text no. 24
Exposito MR, Sanchez IP, Moreno P, Moreno MA, editors. Debut Diabetes Characteristics. 19th European Congress of Endocrinology. Portugal: BioScientifica; 2017.  Back to cited text no. 25
Prudente S, Jungtrakoon P, Marucci A, Ludovico O, Buranasupkajorn P, Mazza T, et al. Loss-of-Function Mutations in APPL1 in Familial Diabetes Mellitus. Am J Hum Genet 2015;97:177-85.  Back to cited text no. 26
Kim SH. Maturity-onset diabetes of the young: What do clinicians need to know? Diabetes Metab J 2015;39:468-77.  Back to cited text no. 27
Johansson BB, Torsvik J, Bjørkhaug L, Vesterhus M, Ragvin A, Tjora E, et al. Diabetes and pancreatic exocrine dysfunction due to mutations in the carboxyl ester lipase gene-maturity onset diabetes of the young (CEL-MODY): A protein misfolding disease. J Biol Chem 2011;286:34593-605.  Back to cited text no. 28
Lombardo D. Bile salt-dependent lipase: Its pathophysiological implications. Biochim Biophys Acta 2001;1533:1-28.  Back to cited text no. 29
Nilsson J, Bläckberg L, Carlsson P, Enerbäck S, Hernell O, Bjursell G. cDNA cloning of human-milk bile-salt-stimulated lipase and evidence for its identity to pancreatic carboxylic ester hydrolase. Eur J Biochem 1990;192:543-50.  Back to cited text no. 30
Lombardo D, Guy O. Studies on the substrate specificity of a carboxyl ester hydrolase from human pancreatic juice. II. Action on cholesterol esters and lipid-soluble vitamin esters. Biochim Biophys Acta 1980;611:147-55.  Back to cited text no. 31
Roudani S, Miralles F, Margotat A, Escribano MJ, Lombardo D. Bile salt-dependent lipase transcripts in human fetal tissues. Biochim Biophys Acta 1995;1264:141-50.  Back to cited text no. 32
Lombardo D, Silvy F, Crenon I, Martinez E, Collignon A, Beraud E, et al. Pancreatic adenocarcinoma, chronic pancreatitis, and MODY-8 diabetes: Is bile salt-dependent lipase (or carboxyl ester lipase) at the crossroads of pancreatic pathologies? Oncotarget 2018;9:12513-33.  Back to cited text no. 33
Johansson BB, Fjeld K, El Jellas K, Gravdal A, Dalva M, Tjora E, et al. The role of the carboxyl ester lipase (CEL) gene in pancreatic disease. Pancreatology 2018;18:12-9.  Back to cited text no. 34
Xiao X, Jones G, Sevilla WA, Stolz DB, Magee KE, Haughney M, et al. A carboxyl ester lipase (CEL) mutant causes chronic pancreatitis by forming intracellular aggregates that activate apoptosis. J Biol Chem 2017;292:7744.  Back to cited text no. 35
Vesterhus M, Raeder H, Kurpad AJ, Kawamori D, Molven A, Kulkarni RN, et al. Pancreatic function in carboxyl-ester lipase knockout mice. Pancreatology 2010;10:467-76.  Back to cited text no. 36
Bläckberg L, Lombardo D, Hernell O, Guy O, Olivecrona T. Bile salt-stimulated lipase in human milk and carboxyl ester hydrolase in pancreatic juice: Are they identical enzymes? FEBS Lett 1981;136:284-8.  Back to cited text no. 37
Ming-Qiang Z, Yang-Li D, Ke H, Wei W, Jun-Fen F, Chao-Chun Z, et al. Maturity onset diabetes of the young (MODY) in Chinese children: Genes and clinical phenotypes. J Pediatr Endocrinol Metab 2019;32:759-65.  Back to cited text no. 38
Moghbeli M, Naghibzadeh B, Ghahraman M, Fatemi S, Taghavi M, Vakili R, et al. Mutations in HNF1A Gene are not a Common Cause of Familial Young-Onset Diabetes in Iran. Indian J Clin Biochem 2018;33:91-5.  Back to cited text no. 39
Mohammadi A, Eskandari A, Sarmadi A, Rahimi M, Iraj B, Hashemipour M, et al. Genetic Study of Hepatocyte Nuclear Factor 1 Alpha Variants in Development of Early-Onset Diabetes Type 2 and Maturity-Onset Diabetes of the Young 3 in Iran. Adv Biomed Res 2019;8:55.  Back to cited text no. 40
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Zhang M, Zhou JJ, Cui W, Li Y, Yang P, Chen X, et al. Molecular and phenotypic characteristics of maturity-onset diabetes of the young compared with early onset type 2 diabetes in China. J Diabetes 2015;7:858-63.  Back to cited text no. 41
George DC, Chakraborty C, Haneef SA, Nagasundaram N, Chen L, Zhu H. Evolution- and structure-based computational strategy reveals the impact of deleterious missense mutations on MODY 2 (maturity-onset diabetes of the young, type 2). Theranostics 2014;4:366-85.  Back to cited text no. 42
Wakil SM, Muiya NP, Tahir AI, Al-Najai M, Baz B, Andres E, et al. A new susceptibility locus for myocardial infarction, hypertension, type 2 diabetes mellitus, and dyslipidemia on chromosome 12q24. Dis Markers 2014;2014:291419.  Back to cited text no. 43
Chapla A, Mruthyunjaya MD, Asha HS, Varghese D, Varshney M, Vasan SK, et al. Maturity onset diabetes of the young in India-a distinctive mutation pattern identified through targeted next-generation sequencing. Clin Endocrinol (Oxf) 2015;82:533-42.  Back to cited text no. 44


  [Figure 1], [Figure 2]

  [Table 1], [Table 2], [Table 3], [Table 4]


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