Prevalence of Helicobacter pylori vacA different genotypes in Isfahan, Iran
S Asghar Havaei1, Parviz Mohajeri2, Reza Khashei3, Rasoul Salehi4, Hamid Tavakoli5
1 Department of Microbiology, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran
2 Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
3 Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
4 Department of Cell Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
5 Department of Gastroenterology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
S Asghar Havaei
Department of Microbiology, Faculty of Medicine, Isfahan University of Medical Science, Hezarjerib St, Isfahan
Source of Support: This research was supported by grant No. 82078 of
Isfahan University of Medical Sciences, Conflict of Interest: None
Background: It is believed that the Helicobacter pylori (H. pylori) vacA gene, as a major virulence determinant (One of the major virulence determinant, not major), may be a risk factor for the development of gastroduodenal diseases. The frequency of vacA genotypes varies in different human populations. This study evaluated the prevalence of vacA alleles/genotypes among dyspeptic patients in Isfahan.
Materials and Methods: One-hundred H. pylori-positive adult patients were examined in this study. After culture of gastric biopsies and DNA extraction from individual H. pylori isolates, the (all H. pylori strains harbor vacA alleles, please replace "presence" with "genotypes") of the vacA s and m alleles were determined using polymerase chain reaction (PCR).
Results: There were four vacA mosaicisms, including 28 for s1a/m1 (28%), 23 for s1b/m1 (23%), 26 for s1a/m2 (26%) and 23 for s1b/m2 (23%). The s2 allele was not found. The predominant vacA genotype in patients with non-ulcer dyspepsia and duodenal ulcer was s1a/m2, whereas in patients with adenocarcinoma was s1a/m1.
Conclusion: The results showed there was no significant correlation between different genotypes of the vacA and the clinical outcomes and appears to vacA genotypes were not useful determinants for gastrointestinal diseases in our area.