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  Indian J Med Microbiol
 

Figure 2: Expression of recombinant Top10 Escherichia coli cells. (a) Glycine-sodium dodecyl sulfate 12% acrylamide gel, (b) Western blot. (a1) Top10 Escherichia coli carrying pBAD vector. (a2-a4): Induced recombinant Top10 Escherichia coli carrying pBAD vector and PlpE-Total (42 and 45 kD). (a5) Not induced Top10 Escherichia coli carrying pBAD vector. (a6-a8) Induced recombinant Top10 Escherichia coli carrying pBAD vector and PlpE1 + 2 + 3 ( 33 kD). (b1) Induced recombinant Top10 Escherichia coli carrying pBAD vector and PlpE1 + 2 + 3. (b2) Induced recombinant Top10 Escherichia coli carrying pBAD vector and PlpE-Total. (a9 and b3) Prestained protein marker

Figure 2: Expression of recombinant Top10 <i>Escherichia coli </i> cells. (a) Glycine-sodium dodecyl sulfate 12% acrylamide gel, (b) Western blot. (a1) Top10 <i>Escherichia coli</i> carrying pBAD vector. (a2-a4): Induced recombinant Top10 <i>Escherichia coli</i> carrying pBAD vector and <i>PlpE-Total </i>(42 and 45 kD). (a5) Not induced Top10 <i>Escherichia coli </i> carrying pBAD vector. (a6-a8) Induced recombinant Top10 <i>Escherichia coli</i> carrying pBAD vector and <i>PlpE1 + 2 + 3 </i><i>(</i> 33 kD). (b1) Induced recombinant Top10 <i>Escherichia coli</i> carrying pBAD vector and <i>PlpE1 + 2 + 3</i>. (b2) Induced recombinant Top10 <i>Escherichia coli </i> carrying pBAD vector and <i>PlpE-Total</i>. (a9 and b3) Prestained protein marker