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  Indian J Med Microbiol
 

Figure 3: Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis of granulocyte-macrophage colony-stimulating factor expression in Escherichia coli BL21(DE3) containing pET28a-granulocyte-macrophage colony-stimulating factor plasmid (cytoplasmic construct) (lane A and B) and Escherichia coli BL21(DE3) containing pET-22b-granulocyte-macrophage colony-stimulating factor plasmid (periplasmic construct) (lane C and D). Lanes A and E belong to total granulocyte-macrophage colony-stimulating factor, and lanes B and D belong to soluble granulocyte-macrophage colony-stimulating factor. M: protein marker. The processed and unprocessed proteins are shown by arrows

Figure 3: Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis of granulocyte-macrophage colony-stimulating factor expression in <i>Escherichia coli</i> BL21(DE3) containing pET28a-granulocyte-macrophage colony-stimulating factor plasmid (cytoplasmic construct) (lane A and B) and <i>Escherichia coli</i> BL21(DE3) containing pET-22b-granulocyte-macrophage colony-stimulating factor plasmid (periplasmic construct) (lane C and D). Lanes A and E belong to total granulocyte-macrophage colony-stimulating factor, and lanes B and D belong to soluble granulocyte-macrophage colony-stimulating factor. M: protein marker. The processed and unprocessed proteins are shown by arrows