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  Indian J Med Microbiol
 

Figure 1: Schematic diagram of the pET22-granulocyte-macrophage colony-stimulating factor expression vector. The positions of enzymatic cleavages are indicated by the red arrow. The gene encoding granulocyte-macrophage colony-stimulating factor protein was inserted into the pET22b vector under the control of the T7 promoter, in frame with a pelB signal peptide and an hexa-histidine tag

Figure 1: Schematic diagram of the pET22-granulocyte-macrophage colony-stimulating factor expression vector. The positions of enzymatic cleavages are indicated by the red arrow. The gene encoding granulocyte-macrophage colony-stimulating factor protein was inserted into the pET22b vector under the control of the <b>T7</b> promoter, in frame with a <i>pelB</i> signal peptide and an hexa-histidine tag