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  Indian J Med Microbiol
 

Figure 1: Isolation of apigenin (A) and caffeic acid (C) from Cousinia verbascifolia by implementing recycle high-performance liquid chromatography technique in the purification process. Fraction Fr.b8d was injected into the recycle high-performance liquid chromatography system using YMC-SIL column (10 μm, 25 cm × 2.5 cm) using chloroform:methanol (85:15) at 250 nm. After three recycles, the peaks were sufficiently resolved to compounds A, B, and C

Figure 1: Isolation of apigenin (A) and caffeic acid (C) from <i>Cousinia verbascifolia</i> by implementing recycle high-performance liquid chromatography technique in the purification process. Fraction Fr.b8d was injected into the recycle high-performance liquid chromatography system using YMC-SIL column (10 μm, 25 cm × 2.5 cm) using chloroform:methanol (85:15) at 250 nm. After three recycles, the peaks were sufficiently resolved to compounds A, B, and C