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  Indian J Med Microbiol
 

Figure 2: In vitro expression analysis of pCHCORE plasmid by western blotting. Assessment of the expression for HBsAg-HCVcp fusion protein in cell lysates of pCHCORE transfected HEK 293T cells was carried out by using anti-HBsAg polyclonal antibody. In lane 1 of the blotted membrane presentation of the doublet bands in expected sizes (≈50 kDa) (indicated arrows) are due to the presence of both glycosylated and nonglycosylated forms of HBsAg-HCVcp fusion protein. Lane 2: Protein MW marker

Figure 2: <i>In vitro</i> expression analysis of pCHCORE plasmid by
western blotting. Assessment of the expression for HBsAg-HCVcp
fusion protein in cell lysates of pCHCORE transfected HEK 293T cells
was carried out by using anti-HBsAg polyclonal antibody. In lane 1 of
the blotted membrane presentation of the doublet bands in expected
sizes (≈50 kDa) (indicated arrows) are due to the presence of both
glycosylated and nonglycosylated forms of HBsAg-HCVcp fusion
protein. Lane 2: Protein MW marker