Close
  Indian J Med Microbiol
 

Figure 1: Schematic presentation of pCHBS and pCHCORE vector construction. (a) In a previous study[11] the HBsAg was PCR amplifi ed and cloned into HindIII/BamHI restriction enzyme sites of pCDNA3.1 to produce pCHBS vector. K/ATG denotes Kozak/start codon. PCMV and BGH pA show the CMV promoter and bovine growth hormone polyadenylation site, respectively. SV40 ori and SV40 pA denote to the same elements from SV40. (b) Schematic diagram for insertion of HCVcp (2-120) DNA sequence in pCHBS (HBsAg harboring pCDNA3.1 plasmid) to construct the pCHCORE vector

Figure 1: Schematic presentation of pCHBS and pCHCORE vector construction. (a) In a previous study[11] the HBsAg was PCR amplifi ed and cloned into <i>Hind</i>III/<i>BamH</i>I restriction enzyme sites of pCDNA3.1 to produce pCHBS vector. K/ATG denotes Kozak/start codon. PCMV and BGH pA show the CMV promoter and bovine growth hormone polyadenylation site, respectively. SV40 ori and SV40 pA denote to the same elements from SV40. (b) Schematic diagram for insertion of HCVcp (2-120) DNA sequence in pCHBS (HBsAg harboring pCDNA3.1 plasmid) to construct the pCHCORE vector