Advanced Biomedical Research

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 9  |  Issue : 1  |  Page : 5-

Anti-Toxoplasma activities of the hydroalcoholic extract of some brassicaceae species


Mahbobeh Montazeri1, Fatemeh Mirzaee2, Ahmad Daryani3, Raheleh Naeimayi4, Shohre Moradi Karimabad4, Hadiseh Khalilzadeh Arjmandi4, Niusha Esmaealzadeh4, Somayeh Shahani6 
1 Toxoplasmosis Research Center; Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran
2 Student Research Committee; Department of Pharmacognosy and Biotechnology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
3 Toxoplasmosis Research Center; Department of Parasitology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
4 Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran

Correspondence Address:
Dr. Somayeh Shahani
Department of Pharmacognosy and Biotechnology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, PC 48471-93698, Sari
Iran

Background: Toxoplasma gondii (T. gondii) is a protozoan parasite that infects a wide range of warm-blooded animals and humans. The conventional anti-Toxoplasma treatments cause significant toxicity. Brassicaceae family contains several medicinal plants with anti-inflammatory, chemopreventive, insecticide, antibacterial, antiviral, and antiparasitic effects. In this study, the hydroalcoholic extract of some Brassicaceae species was investigated against T. gondii in vitro. Materials and Methods: Seeds of Alyssum homolocarpum, Lepidium perfoliatum, Lepidium sativum, and aerial parts of Nasturtium officinale and Capsella bursa-pastoris were extracted by maceration method using 80% ethanol. Vero cells were treated with different concentrations (5–600 μg/mL) of the extracts and pyrimethamine (as positive control), and the cellular viability was verified. Next, Vero cells were infected by T. gondii tachyzoites (RH strain), and the viability of the infected cells was measured by a colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Results: The 50% inhibitory concentration values were 5.1, 14.67, 32.49, 37.31, 71.35, and 2.63 μg/mL, and the selectivity indices were 8.06, 2.59, 0.74, 0.78, 0.65 (P < 0.05 compared with positive control), and 3.03 for L. sativum, L. perfoliatum, N. officinale, A. homolocarpum, C. bursa-pastoris, and pyrimethamine, respectively. Conclusion: The results of this study demonstrated that the hydroalcoholic extracts of L. sativum and L. perfoliatum have the promising anti-Toxoplasma activity by growth inhibition of T. gondii tachyzoites in infected cells.


How to cite this article:
Montazeri M, Mirzaee F, Daryani A, Naeimayi R, Moradi Karimabad S, Khalilzadeh Arjmandi H, Esmaealzadeh N, Shahani S. Anti-Toxoplasma activities of the hydroalcoholic extract of some brassicaceae species.Adv Biomed Res 2020;9:5-5


How to cite this URL:
Montazeri M, Mirzaee F, Daryani A, Naeimayi R, Moradi Karimabad S, Khalilzadeh Arjmandi H, Esmaealzadeh N, Shahani S. Anti-Toxoplasma activities of the hydroalcoholic extract of some brassicaceae species. Adv Biomed Res [serial online] 2020 [cited 2020 Sep 30 ];9:5-5
Available from: http://www.advbiores.net/article.asp?issn=2277-9175;year=2020;volume=9;issue=1;spage=5;epage=5;aulast=Montazeri;type=0