Advanced Biomedical Research

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 8  |  Issue : 1  |  Page : 41-

Renal Tissue Damages and Its Antioxidant Status Improved by Crab Shell Extract in Streptozotocin-induced Diabetic Rat


Zohreh Ebrahimi1, Mohammad Rasool Khazaei2, Elham Ghanbari2, Mozafar Khazaei2 
1 Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran
2 Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran

Correspondence Address:
Dr. Mozafar Khazaei
Fertility and Infertility Research Center, Medical School, University Ave., Shahid Shirodi Blvd., Kermanshah
Iran

Background: Diabetic nephropathy is a complex and multifactorial adverse effect of diabetes mellitus (DM). Crab shell as a natural product is supposed to have antioxidant effect which is one of the important mechanisms to improve DM. The aim of this study was to investigate the effect of crab shell extract (CSE) on the histopathology and antioxidant status of kidney in diabetic rats. Materials and Methods: Forty-two adult Wistar rats (210 ± 10 g) were divided into six groups (n = 7). Streptozotocin (50 mg/kg) was administered interaperitonealy (IP) for inducing diabetes. Rats were treated for 14 days by CSE with 100, 200, and 400 mg/kg doses IP. Fasting blood glucose, body, and renal weight were evaluated. The antioxidant status of kidney's tissue was evaluated by determining the level of ferric-reducing antioxidant power (FRAP). Furthermore, urine samples were used to determine nitric oxide (NO) levels. Microscopic slides were prepared to compare kidney histology between groups. Data were analyzed by one-way analysis of variance with post hoc Tukey's test, and P < 0.05 was considered statistically significant. Results: CSE induced a significant reduction in blood glucose (P = 0.01) and a significant increase in total antioxidant capacity (FRAP) (P = 0.004). Furthermore, urine NO was decreased significantly (P = 0.000). The extract improved renal tissue changes caused by diabetes. Conclusion: CSE improved antioxidant status and diabetic histological changes of rat kidney, and it could be an alternative complementary therapy in diabetic-associated disorders.


How to cite this article:
Ebrahimi Z, Khazaei MR, Ghanbari E, Khazaei M. Renal Tissue Damages and Its Antioxidant Status Improved by Crab Shell Extract in Streptozotocin-induced Diabetic Rat.Adv Biomed Res 2019;8:41-41


How to cite this URL:
Ebrahimi Z, Khazaei MR, Ghanbari E, Khazaei M. Renal Tissue Damages and Its Antioxidant Status Improved by Crab Shell Extract in Streptozotocin-induced Diabetic Rat. Adv Biomed Res [serial online] 2019 [cited 2019 Dec 11 ];8:41-41
Available from: http://www.advbiores.net/article.asp?issn=2277-9175;year=2019;volume=8;issue=1;spage=41;epage=41;aulast=Ebrahimi;type=0