Advanced Biomedical Research

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 8  |  Issue : 1  |  Page : 40-

Microsatellite Marker Analysis for Laboratory Mice Profiling


Reza Ahangari Cohan1, Davoud Nouri Inanlou2, Mohammad Hasan Samiee Aref3, Sirous Zeinali4, Ramin Farhoudi2 
1 Department of Nanobiotechnology, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran
2 Department of Quality Control, Pasteur Institute of Iran, Tehran, Iran
3 Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran
4 Department of Molecular Medicine, Pasteur Institute of Iran, Tehran, Iran

Correspondence Address:
Dr. Ramin Farhoudi
Department of Quality Control, Pasteur Institute of Iran, Tehran
Iran

Background: The key point in the production procedure of inbred animals is checking the genetic purity. Skin grafting and coat color test are used traditionally to prove genetic purity, but they have some disadvantages. Recent advances in DNA profiling have enabled scientists to check easily the genetic purity of laboratory animals. In the current study, a set of microsatellite markers was designed to check the purity of inbred laboratory mice. Materials and Methods: Twenty microsatellites located on 20 chromosomes were employed to create a distinctive genetic profile for parentage analysis. Each individual primer was designed based on distinguishable colors and separable sizes. Results: Twenty specific microsatellite markers were used in the polymerase chain reaction mixture to identify inbred BALB/cJ strains. Our results confirmed that the designed microsatellites are excellent genetic markers for testing inbred BALB/cJ strain in laboratories. Conclusion: Our study showed that genetic profiling using microsatellite markers allows us to detect the genetic differences of laboratory mouse species in quality control tests and validation steps.


How to cite this article:
Ahangari Cohan R, Nouri Inanlou D, Samiee Aref MH, Zeinali S, Farhoudi R. Microsatellite Marker Analysis for Laboratory Mice Profiling.Adv Biomed Res 2019;8:40-40


How to cite this URL:
Ahangari Cohan R, Nouri Inanlou D, Samiee Aref MH, Zeinali S, Farhoudi R. Microsatellite Marker Analysis for Laboratory Mice Profiling. Adv Biomed Res [serial online] 2019 [cited 2019 Nov 20 ];8:40-40
Available from: http://www.advbiores.net/article.asp?issn=2277-9175;year=2019;volume=8;issue=1;spage=40;epage=40;aulast=Ahangari;type=0