Advanced Biomedical Research

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 6  |  Issue : 1  |  Page : 6-

Differential Immune Reactivity Pattern of SW48 and SW1116 Colorectal Cancer Cell Lines with Colorectal Cancer Patients Sera


Ghasem Ghalamfarsa1, Seyyed Vahid Hosseini2, Maryam Hamidinia4, Abbas Ghaderi4, Mahmoud Mahmoudi3, Zahra Mojtahedi5 
1 Department of Immunology and Allergy, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad; Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2 Colorectal Research Center, Faghihi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran
3 Department of Immunology and Allergy, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Correspondence Address:
Dr. Zahra Mojtahedi
Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz
Iran

Background: Colorectal cancer (CRC) is one of the most commonly diagnosed cancers worldwide. It is also known as the second leading cause of deaths as the early stage detection is not yet available by current methods. So identification of biomarkers can also be functional in early diagnosis and prognosis. Materials and Methods: We examined sera from 60 CRC patients of different stages as a source of auto-antibody as well as two human CRC cell lines with different invasive capacities (SW48 and SW1116) as the source of antigens. The pattern of immune reactivity in immuneblotting tests between mentioned cell lines and CRC patients' sera were evaluated by ImageJ software. Results: The Immune reactivity pattern of two cell lines (SW48 and SW1116) with CRC patients' sera were different in band intensities and the most immune reactivity intensity was observed in SW48 cell lysate with sera from Stage III CRC patients. Conclusion: Due to the humoral immune response, sera from Stage III CRC patients contained autoantibodies that demonstrated higher immune reactivity. Moreover, SW48 cell line with high aggressive behavior reacted to CRC patients' sera with greater intensity compared with less aggressive behavior cell line (SW1116). Therefore, it is required to use other techniques such as two-dimensional electrophoresis and mass spectrometry.


How to cite this article:
Ghalamfarsa G, Hosseini SV, Hamidinia M, Ghaderi A, Mahmoudi M, Mojtahedi Z. Differential Immune Reactivity Pattern of SW48 and SW1116 Colorectal Cancer Cell Lines with Colorectal Cancer Patients Sera.Adv Biomed Res 2017;6:6-6


How to cite this URL:
Ghalamfarsa G, Hosseini SV, Hamidinia M, Ghaderi A, Mahmoudi M, Mojtahedi Z. Differential Immune Reactivity Pattern of SW48 and SW1116 Colorectal Cancer Cell Lines with Colorectal Cancer Patients Sera. Adv Biomed Res [serial online] 2017 [cited 2020 Mar 28 ];6:6-6
Available from: http://www.advbiores.net/article.asp?issn=2277-9175;year=2017;volume=6;issue=1;spage=6;epage=6;aulast=Ghalamfarsa;type=0