Advanced Biomedical Research

BRIEF REPORT
Year
: 2015  |  Volume : 4  |  Issue : 1  |  Page : 48-

Detection of Legionella spp. by a nested-PCR assay in air samples of a wastewater treatment plant and downwind distances in Isfahan


Seyyed Abbas Mirzaee1, Mahnaz Nikaeen1, Yaghob Hajizadeh1, BiBi Fatemeh Nabavi1, Akbar Hassanzadeh2 
1 Department of Environmental Health Engineering and Environment Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
2 Department of Statistics and Epidemiology, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran

Correspondence Address:
Mahnaz Nikaeen
Department of Environmental Health Engineering, School of Health, Isfahan University of Medical Sciences, Hezar Jerib Avenue, Isfahan
Iran

Background: Wastewater contains a variety of pathogens and bio -aerosols generated during the wastewater treatment process, which could be a potential health risk for exposed individuals. This study was carried out to detect Legionella spp. in the bio -aerosols generated from different processes of a wastewater treatment plant (WWTP) in Isfahan, Iran, and the downwind distances. Materials and Methods: A total of 54 air samples were collected and analyzed for the presence of Legionella spp. by a nested- polymerase chain reaction (PCR) assay. A liquid impingement biosampler was used to capture bio -aerosols. The weather conditions were also recorded. Results: Legionella were detected in 6% of the samples, including air samples above the aeration tank (1/9), belt filter press (1/9), and 250 m downwind (1/9). Conclusion: The result of this study revealed the presence of Legionella spp. in air samples of a WWTP and downwind distance, which consequently represent a potential health risk to the exposed individuals.


How to cite this article:
Mirzaee SA, Nikaeen M, Hajizadeh Y, Nabavi BF, Hassanzadeh A. Detection of Legionella spp. by a nested-PCR assay in air samples of a wastewater treatment plant and downwind distances in Isfahan.Adv Biomed Res 2015;4:48-48


How to cite this URL:
Mirzaee SA, Nikaeen M, Hajizadeh Y, Nabavi BF, Hassanzadeh A. Detection of Legionella spp. by a nested-PCR assay in air samples of a wastewater treatment plant and downwind distances in Isfahan. Adv Biomed Res [serial online] 2015 [cited 2020 Jun 1 ];4:48-48
Available from: http://www.advbiores.net/article.asp?issn=2277-9175;year=2015;volume=4;issue=1;spage=48;epage=48;aulast=Mirzaee;type=0