Year : 2015 | Volume
: 4 | Issue : 1 | Page : 1-
Association of KCNJ11 (E23K) gene polymorphism with susceptibility to type 2 diabetes in Iranian patients
Ali Rastegari1, Mohammad Rabbani1, Hamid Mirmohammad Sadeghi1, Elham Faghih Imani2, Akbar Hasanzadeh3, Fatemeh Moazen1
1 Department of Pharmaceutical Biotechnology, University of Medical Sciences, Isfahan, Iran
2 Department of Internal Medicine, University of Medical Sciences, Isfahan, Iran
3 Department of Biostatistics and Epidemiology, University of Medical Sciences, Isfahan, Iran
Background: Type 2 diabetes (T2D) is a multifactorial disease with susceptibility of several genes that are related to T2D. Insulin secretion pathway starts with potassium channels in pancreatic beta cells. KCNJ11 gene encodes ATP-sensitive potassium channel subunits. Some studies suggested that KCNJ11 (E23K) mutation increases the risk of T2D. Therefore, present study was designed to investigate the association between E23K polymorphism of KCNJ11 gene and type 2 diabetes mellitus (T2DM) in the Iranian population.
Materials and Methods: The type of study was case-control and 40 unrelated subjects, including 20 healthy controls and 20 diabetic patients were recruited (diagnosed based on American Diabetes Association criteria). Blood samples were used for isolation of genomic deoxyribonucleic acid (DNA). Having extracted the genomic DNA from human blood leukocytes by means of High Pure PCR Template Preparation Kit, PCR-restriction fragment length polymorphism method was used to detect KCNJ11 E23K gene polymorphism. BanII restriction enzyme was used for digestion. Data were analyzed using Chi-square or Fisher exact test or independent t-test, as appropriate. P < 0.05 was considered.
Results: We found that the carrier homozygous for KK genotype are susceptible to T2D (0.049) and in patients the frequency of K allele was higher than control subjects (0.048).
Conclusion: The present study suggests that KCNJ11 (E23K) gene polymorphism is associated with T2DM.
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan
|How to cite this article:|
Rastegari A, Rabbani M, Sadeghi HM, Imani EF, Hasanzadeh A, Moazen F. Association of KCNJ11 (E23K) gene polymorphism with susceptibility to type 2 diabetes in Iranian patients.Adv Biomed Res 2015;4:1-1
|How to cite this URL:|
Rastegari A, Rabbani M, Sadeghi HM, Imani EF, Hasanzadeh A, Moazen F. Association of KCNJ11 (E23K) gene polymorphism with susceptibility to type 2 diabetes in Iranian patients. Adv Biomed Res [serial online] 2015 [cited 2020 Mar 28 ];4:1-1
Available from: http://www.advbiores.net/article.asp?issn=2277-9175;year=2015;volume=4;issue=1;spage=1;epage=1;aulast=Rastegari;type=0