Advanced Biomedical Research

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 3  |  Issue : 1  |  Page : 167-

Identification of Leishmania isolates from Varzaneh city, Isfahan province, Iran using nested polymerase chain reaction method


Reza Arjmand1, Sedigheh Saberi1, Sepideh Tolouei1, Zahra Chizari1, Reza Fadaei Nobari2, Simindokht Soleimani Fard1, Mojtaba Akbari3, Seyed Hossein Hejazi4 
1 Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 CDC Manager in Isfahan Health Deputy, Isfahan University of Medical Sciences, Isfahan, Iran
3 Department of Research, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4 Department of Parasitology and Mycology, School of Medicine; Skin Disease and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Correspondence Address:
Seyed Hossein Hejazi
Skin Disease and Leishmaniasis Research Center, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan
Iran

Background: Leishmaniasis, a parasitic disease, is caused by the Leishmania genus, a protozoan parasite transmitted by sand fly arthropods. Cutaneous leishmaniasis (CL) in old world is usually caused by L. major, L. tropica, and L. aethiopica complexes. One of the most important hyper endemic areas of CL in Iran is Isfahan province. Varzaneh is a city in the eastern part of Isfahan province. Due to different biological patterns of parasite strains which are distributed in the region, this study was design to identify Leishmania species from human victims using Kinetoplastid DNA as templates in a molecular PCR method. Materials and Methods: Among 186 suspected cases, 50 cases were confirmed positive by direct microscopy after Giemsa staining. Species characterization of the isolates was done using Nested- PCR as a very effective and sensitive tool to reproduce mini circle strands. Results: After Nested-PCR from all 50 cases, 560 bp bands were produced which according to products of reference strains indicate that the infection etiologic agent has been L. major. 22 (44%) of patients were females and 28 (56%) of them were males. Their age ranges were between 7 months and 60 years. Conclusion: According to the results of the study and the particular pattern of infection prevalent in the region, genetic studies and identification of Leishmania parasites are very important in the disease control and improvement of regional strategy of therapy protocols.


How to cite this article:
Arjmand R, Saberi S, Tolouei S, Chizari Z, Nobari RF, Fard SS, Akbari M, Hejazi SH. Identification of Leishmania isolates from Varzaneh city, Isfahan province, Iran using nested polymerase chain reaction method.Adv Biomed Res 2014;3:167-167


How to cite this URL:
Arjmand R, Saberi S, Tolouei S, Chizari Z, Nobari RF, Fard SS, Akbari M, Hejazi SH. Identification of Leishmania isolates from Varzaneh city, Isfahan province, Iran using nested polymerase chain reaction method. Adv Biomed Res [serial online] 2014 [cited 2020 Aug 15 ];3:167-167
Available from: http://www.advbiores.net/article.asp?issn=2277-9175;year=2014;volume=3;issue=1;spage=167;epage=167;aulast=Arjmand;type=0