Comparison of the frequency of Y-short tandem repeats markers between Sadat and non-Sadat populations in Isfahan province of Iran
Reihaneh Seyedebrahimi1, Ebrahim Esfandiari1, Bahman Rashidi1, Rasoul Salehi2, Ali Gholami Dahghi3, Shahriar Dabiri4, Majid Kheirollahi2
1 Department of Anatomy, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences; Pediatric Inherited Diseases Research Center, Research Institute for Primordial Prevention of Noncommunicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran
3 Department of Islamic Studies, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4 Department of Pathology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Department of Genetics and Molecular Biology, Pediatric Inherited Diseases Research Center, School of Medicine, Isfahan University of Medical Sciences, P.O. Box 81746-73461, Isfahan
Source of Support: None, Conflict of Interest: None
Background: Y chromosome is one of the two sex chromosomes and is male specific. Due to limited genetic exchange, the main part of that is passed virtually unchanged from one generation to next generation. The short tandem repeats (STRs) are almost constant on chromosomes that make them as an appropriate factor for use in population genetic studies. In this study, we used the STRs of Y chromosome markers in Sadat families and comparison with other families was investigated.
Materials and Methods: In this study, sampling was done from fifty unrelated males of Sadat families and fifty unrelated males of non-Sadat families. After the extraction of DNA from blood samples and primer design, polymerase chain reaction (PCR) was performed for each primer pairs separately. The PCR products were run on agarose gel that followed by running on polyacrylamide gel for better resolution. In addition, some sequenced samples were used as identified markers to determine the length of other alleles in polyacrylamide gel.
Results: The survey of six STR in two case and control groups was carried out, and analysis revealed that the frequency of some alleles is different in case group compared to control group. Allele frequency of the markers DYS392, DYS393, DYS19, DYS390, DYS388, and DYS437 on the Y chromosome in Sadat families was quite different in comparison with other families.
Conclusions: The reason for these differences in allele frequencies of the Sadat family in comparison with other families is having a common ancestor.