Expression of CXC Chemokine Receptors in Acute Ulcerative Colitis: Initial Study from an Animal Model
Poorya Foroutan1, Mohammad Amin Boshagh1, Mohammad Raman Moloudi2, Shohreh Fakhari2, Bahram Nikkhoo2, Ali Jalili1
1 Cancer and Immunology Research Center; Department of Immunology and Hematology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
2 Cancer and Immunology Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran
|Date of Web Publication||23-Sep-2019|
Dr. Ali Jalili
Cancer and Immunology Research Center, Kurdistan University of Medical Sciences, Sanandaj
Source of Support: None, Conflict of Interest: None
Background: Ulcerative colitis (UC) is an inflammatory disease which is characterized by infiltration of inflammatory cells, crypt abscesses, distortion of the mucosal glands, and goblet cell depletion. The existence of neutrophil-rich inflammation in colon tissues of patients with UC is one of the most significant histological features of this disease. Nonetheless, the expression of CXCR chemokine receptors which appear as the main chemical mediators governing the migration of neutrophils into the mucosal tissue of patients with UC has not been well clarified. Materials and Methods: In this experimental study, the UC model was induced in Wistar rats by administration of 2 ml 4% acetic acid into the large colon through the rectum. Animals were anesthetized after 48 h; their colon tissue samples were isolated for macroscopic and histopathological examination. The expression of receptor1-7of CXC chemokine was assessed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) technique. Results: Heavy infiltration of neutrophils, coagulative necrosis, and ulcers were observed in H and E staining, which pathologically proved the UC model. qRT-PCR results indicated that CXCR2 as one of the important ELR+ chemokine family receptors bears the highest expression in the UC group (32 fold) than the control group (P ≤ 0.05). In addition, other CXCRs of this group including CXCR1 did not possess any change (P > 0.05). In contrast, RLR negative chemokine family receptors did not show any changes with the normal group. Conclusion: The results showed that CXCR2 is the only receptor for CXCL family which was remarkably upregulated in experimental UC and that CXCR2 might play a significant role in the pathogenesis of UC.
Keywords: CXCR, chemokines, inflammation, ulcerative colitis
|How to cite this article:|
Foroutan P, Boshagh MA, Moloudi MR, Fakhari S, Nikkhoo B, Jalili A. Expression of CXC Chemokine Receptors in Acute Ulcerative Colitis: Initial Study from an Animal Model. Adv Biomed Res 2019;8:56
|How to cite this URL:|
Foroutan P, Boshagh MA, Moloudi MR, Fakhari S, Nikkhoo B, Jalili A. Expression of CXC Chemokine Receptors in Acute Ulcerative Colitis: Initial Study from an Animal Model. Adv Biomed Res [serial online] 2019 [cited 2020 Apr 2];8:56. Available from: http://www.advbiores.net/text.asp?2019/8/1/56/267559
| Introduction|| |
Ulcerative colitis (UC) is implied as one of the important causes of gastrointestinal disease. UC is just a pathological condition in which the inflammatory reaction and morphologic modifications are limited to the colon. The rectum seems involved in most of the patients. The typical histologic findings appear to be acute and chronic inflammation of the mucosa by polymorphonuclear cells (PMNs) and mononuclear cells, crypt abscesses, distortion of the mucosal glands, and goblet cell depletion.,,,, The endless existence and trafficking of immunocytes into the mucosal section are called physiologic inflammation and mirror the production of chemokines by cells inside the gastrointestinal mucosa. Trafficking of immunocytes to the site of the inflammation or injury occurred through the interaction of small molecules, called chemokines and chemokine receptors.,,
The chemokines are a superfamily of low molecular weight which serves as effective chemoattractants for inflammatory cells. Chemokines had been ordered into four subfamilies, based on the existence of cysteines at the amino-terminal: CXC, CC, CX3C, and C.,, Through CXC, chemokine can be subclassified based on either the presence or absence of a tripeptide motif ELR at the NH2 terminus into Glu-Leu-Arg (ELR)+ and ELR − CXC family., The characteristic of ELR + chemokines is their ability to recruit PMNs, specifically neutrophils into the inflamed tissues; this subclass contains CXCR2 and CXCR1 that are shown to be the main receptors of ELR + CXC subfamily which are strong chemoattractants for neutrophils., ELR − CXC subfamily includes chemokines such as CXCL9, CXCL10, and CXCL11, and their main receptor is CXCR3 which primarily attracts activated Th1 cells and natural killer cells. CXCR4, CXCR5, CXCR6, and CXCR7 are other members of ELR - subfamily. Although CXCR4 and CXCR7 through binding to their ligand CXCL12 play an important role in trafficking of many cells such as T and B lymphocytes, and hematogenic stem cells, CXCR5 is a chemoattract for B-lymphocytes.,, On the other hand, several studies have shown the importance of CXC chemokines and their receptors in UC, for instance, Farooq et al. showed that blocking of CXCR2 receptor resulted in attenuation of inflammation in an animal model of UC. Moreover, other studies showed that CXCR2- and CXCR3-knockout mice were protected against colitis., Taken together, accumulating evidence indicates that chemokines and their receptors are key players in the inflammatory status of colitis. However, not only the etiology and pathobiology of colitis are unclear but also the expression of all inflammatory mediators, particularly chemokine receptors, in this disease has not well studied. Herein, we examined the expression levels of 1–7 members of CXCR chemokine receptors at mRNA levels in the experimental model of UC and found that CXCR2 appeared the only receptor for ELR + chemokine which was significantly being upregulated within the UC group. In contrast, the expression of entire members of ELR − CXCRs did not significantly change.
| Materials and Methods|| |
Male Wistar rats (300–400 g) were got from Kurdistan University of Medical Sciences. The animals were fed a regular chow pellet regimen, had free admission to water, and were kept on a 12-h light/dark cycle. The experimental protocol was approved (Approval ID: IR.MUK.REC.1397.319) by Kurdistan University of Medical Sciences. In this study, 12 adults male Wistar rats were randomly divided into two groups of control and acetic acid (AA)-induced colitis groups. Experimental colitis was induced in 6 rats by administration of AA and designed as AA-induced colitis group. The other 6 rats which were treated with normal saline designated as the control group. It should be noted that all the rats should be kept for 2–3 months to reach an ideal weight of 300–400 g to reduce their mortality during the test.
Induction of ulcerative colitis and sample preparation
Induced colitis was carried out as described previously. rats were lightly anesthetized with ether, and then, AA 4% (pH = 2.3) was administered into the distal colon through a cannula (2-mm internal diameter and 6-cm long into the colon from the rectum). In order to induce colitis, rats were sacrificed by chloroform after 48 h, then 6 cm of the distal part of colon was removed and used as sample for the molecular and microscopic evaluations. About 2 cm from the mucosal part of the colon tissue was placed in an RNase-free tube and immediately stored at −70°C freezer for mRNA extraction, and the rest of removed colons were fixed in 10% formalin for later histopathological evaluations.
Evaluation of edema
In order to evaluate the amount of edema, 6 cm of the distal part of colon was separated and weighed, then weight/length ratio of wet colon in each group was calculated.
Colonic tissues were fixed overnight in 10% formalin, dehydrated in ethanol, then embedded in paraffin, and sectioned at 5-μm thick. Histological sections were stained with hematoxylin/eosin (H and E) according to the standard protocols and used for the detection of inflammation and verification of the model. To confirm UC, microscopic analysis was performed to study white blood cell (WBC) infiltration, ulcers, and other characters by our pathologist (BN) who was blind to each group.
RNA extraction and quantitative real-time polymerase chain reaction
Total RNA was extracted from the colonic mucosa tissue using RNA isolation kit according to the manufacturer's instructions (#740955.50; NucleoSpin RNA, Germany), and cDNA synthesis was performed for quantitative reverse transcription-polymerase chain reaction (qRT-PCR) using PrimeScript™ RT Reagent Kit (#RR037A; Takara, Japan). Real-time qRT-PCR was performed by Corbett Rotor-Gene 6000 real-time PCR system (Corbett Research, Australia) and carried out using SYBR Green dye detection protocol. The primers were designed by Gene Runner and are listed in [Table 1]. The expression of target genes was quantified using the comparative Cycle Threshold (CT) method. Amplification condition was as follows: initial denaturation at 94°C for 30 s, followed by 40 cycles of denaturation at 95°C for 5s, annealing temperature at 55°C for 30 s, extension temperature at 72°C for 30 s, and final extension temperature at 72°C for 5 min. GAPDH was used as a housekeeping gene, and of course, the expression of CXCR and GAPDH genes was quantified in AA-induced colitis and control groups. We calculated the mean ± standard deviation for each group using 2−△CT [2−(CTCXCR–CTGAPDH)]. Then, the fold change was calculated using this formula: 2−△CT AA-induced colitis group/2−△CT control group.
In this study, SPSS version 20.0, IBM, Chicago and t-test were used to compare the results of gene expressions and colon/body weight ratio of rats in the UC and control groups (P ≤ 0.05).
| Results|| |
Macroscopic evaluation of colon tissue
Macroscopic analysis showed: thickening of the colon, severe hemorrhagic ulcers, and bloody mucosa in AA-induced colitis group. In the other hand, there were no signs of inflammation or tissue damaging in the control group [Figure 1]a and [Figure 1]b. Finally, the whole body weight was measured before sacrificed, and the ratio of the mean wet colon weight to the mean whole body weight was calculated through both the groups and their results, as shown in [Figure 1]c; this ratio was much higher in the AA-induced colitis group than the control group.
|Figure 1: Macroscopic evaluations of the colon tissue from the acetic acid-induced colitis and control groups. (a and b) Gross macroscopic architecture of the colon in the acetic acid-induced colitis and control groups. (c) Colon weight/length ratio through the acetic acid-induced colitis and control groups. This ratio considers as edema, and each column represents fold change + standard error of the mean (SEM) for six male Wistar rats. *(P < 0.05)|
Click here to view
Histopathological evaluations represented epithelial destruction, goblet cells depletion, ulcerated mucosa and infiltration of WBC mostly PMNs in AA-induced colitis [Figure 2]. In the other hand, we can see healthy and intact architecture of the colon tissue without any inflammation through the control group [Figure 3].
|Figure 2: Histological observations from colon biopsies of acetic acid-induced colitis. (a) Goblet cell depletion (×10). (b) Dominant infiltration of neutrophils (×40). (c). Ulcerated tissue and neutrophil infiltration (×10). (d) Epithelial destruction (×10)|
Click here to view
|Figure 3: Histological observation from colon biopsies of the control group. As we can see in this figure, architecture of the colon tissues is solely normal, and there are no ulcer or infiltration of neutrophils (a and b, ×4, c × 40)|
Click here to view
ELR + receptor expression in mucosa of experimental colitis model
Our investigations from quantitative real-time PCR showed the overexpression of CXCR2 in inflamed mucosa of AA-induced colitis, but not in healthy ones (P< 0.05); in fact, results of fold change showed approximately 33 times higher through the AA-induced colitis group rather than the control group. However, unlike CXCR2 expression, we did not find any change in the expression of CXCR1 between the AA-induced group and the control group (P > 0.05) [Figure 4].
|Figure 4: ELR+CXCRs mRNA expression of the mucosa of the colon tissues from biopsies of the acetic acid-induced colitis group and control group. CXCR2 ELR+ CXC chemokine receptor is highly upregulated in the acetic acid-induced colitis group than the control group. Each column represents a fold change ± SEM for six male Wistar rats. The statistical results for CXCR2 in the acetic acid-induced colitis group show a statistically significant difference compared to the control group *(P ≤ 0.05). However, in contrast, CXCR1 remained unchanged in compared through both the groups (P > 0.05)|
Click here to view
ELR − receptor expression through mucosa of experimental colitis model
Our investigations of quantitative real-time PCR showed that CXCR5 relative expression had lightly elevated in inflamed mucosa than healthy ones. Although the levels of CXCR5 expression at mRNA were slightly unregulated (1.4 times) but it was not statistically significant. Other CXCRs such as CXCR3, CXCR4, CXCR6, and CXCR7 mRNA expression in the mucosa of the AA-induced colitis group remained unchanged in comparison with the control group (P > 0.05) [Figure 5].
|Figure 5: ELR− CXCR mRNA expression of the mucosa of the colon tissues from biopsies of the acetic acid-induced colitis group and control group. Each column represents a fold change ± SEM for six male Wistar rats. The statistical results for ELR− CXCRs in the acetic acid-induced colitis group show no significant difference in compared with the control group (P > 0.05)|
Click here to view
| Discussion|| |
Due to the incomplete and unknown etiology of UC and importance of the disease as one of the most important causes of gastrointestinal disorders on the one hand, and on the other hand, the importance of the immune system in etiology of UC, we were about to evaluate mRNA of CXCRs family as an important part of the immune system. Growing of evidence indicates that neutrophils are the most important players in acute inflammation. ELR + C-X-C motif chemokine family is involved in trafficking of neutrophils through the binding of their cognate receptors. CXCL1 and CXCL8 are two important ELR + CXCL family chemokines, and their interaction with CXCR2 leads in the migration of neutrophils., In line with previous studies, we have reported here that CXCR2 is remarkably overexpressed in experimental UC than the control. Based on our observation, we have considered that this model seems an acute induced UC and the main infiltrated cells appear to be neutrophils. In contrast, the expression levels of CXCR1, another receptor for ELR + chemokines, remain unchanged. This discrepancy could be explained by the following reasons:first of all, maybe CXCL8 which is main and high-affinity ligand for CXCR1 does not exist in the rats., Second, previous studies have shown that CXCR1, unlike CXCR2, needs more concentration of ligands to respond. Moreover, CXCR2 can alone activate PMNs, but CXCR1 does not have this ability. Since the sensitivity of CXCR2 is higher that CXCR1 in responding to its ligands, we postulate that this could be another reason explaining the remarkable upregulation of CXCR2 in our model. Finally, from the biological point of view, there are some structural differences between rat CXCRs and human CXCRs, for instance, in terms of homology, there are about 58% of differences between rat CXCR1 and human CXCR1. Based on these facts, the results from human studies and experimental models can vary from each other.
We envision that in our model, some CXCR2-expressing neutrophils may react with CXCR2 ligands, CXCL6 and CXCL3, which have been remarkably upregulated in the colonic tissue (Boshagh et al. in press) and infiltrate into the inflamed colon. Other studies established that anti-CXCR2 mAb decreases neutrophil infiltration as well as the severity of colitis. Accordingly, CXCR2 knockout rats showed that more mild symptoms of UC, such as ulcer and bleeding, will be attenuated.,, Some clinical studies have clearly shown that the levels of CXCL8 increased in the serum of patients with active UC. Consistently, it has been demonstrated that CXCR1 and CXCR3 were upregulated on the cell surface of colonic macrophages and T-lymphocytes in patients with active US. However, in our hands, the expression levels of both CXCR1 and CXCR3 were similar to the control group. Our observations are consistent with the fact that CXCR3 is a receptor of CXCL10 and chemoattracts T-cells.
Herein, we demonstrated the expression of other CXCRs such as CXCR3, CXCR4, CXCR5, CXCR6, and CXCR7 are almost similar in the control group and the UC group. However, Werner et al. reported that the expression levels of CXCR4 in peripheral blood T-cells and colon epithelial cells were higher in patients with UC than the healthy controls. In contrast with the present study which is observed that the expression levels of CXCR6 are similar in both control and experimental UC, a previous study exhibited that the expression of CXCR6 and its ligand CXCL16 was significantly higher in mucosa tissue of patients with Crohn's disease than the healthy controls. This discrepancy could be due to the difference in nature of UC which occurs in human and the experimental models which are induced in the animal models.
| Conclusion|| |
The results of the current study showed that the CXCR2 seems more likely to be the only chemokine receptor which remarkably upregulated in experimental UC and that CXCR2 could be a suitable candidate target for the treatment of UC. However, further studies, especially human studies, are required.
This work was supported by grants from Kurdistan University of Medical Sciences to AJ for supporting of PF's thesis as an MSc student in Medical Immunology at Kurdistan University of Medical Sciences.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Abraham C, Cho JH. Inflammatory bowel disease. N
Engl J Med 2009;361:2066-78.
Yu YR, Rodriguez JR. Clinical presentation of Crohn's, ulcerative colitis, and indeterminate colitis: Symptoms, extraintestinal manifestations, and disease phenotypes. Semin Pediatr Surg 2017;26:349-55.
Raab Y, Gerdin B, Ahlstedt S, Hällgren R. Neutrophil mucosal involvement is accompanied by enhanced local production of interleukin-8 in ulcerative colitis. Gut 1993;34:1203-6.
Matricon J, Barnich N, Ardid D. Immunopathogenesis of inflammatory bowel disease. Self Nonself 2010;1:299-309.
Baumgart DC, Sandborn WJ. Inflammatory bowel disease: Clinical aspects and established and evolving therapies. Lancet 2007;369:1641-57.
Zhang YZ, Li YY. Inflammatory bowel disease: Pathogenesis. World J Gastroenterol 2014;20:91-9.
MacDermott RP, Sanderson IR, Reinecker HC. The central role of chemokines (chemotactic cytokines) in the immunopathogenesis of ulcerative colitis and Crohn's disease. Inflamm Bowel Dis 1998;4:54-67.
Papadakis KA, Targan SR. The role of chemokines and chemokine receptors in mucosal inflammation. Inflamm Bowel Dis 2000;6:303-13.
Zimmerman NP, Vongsa RA, Wendt MK, Dwinell MB. Chemokines and chemokine receptors in mucosal homeostasis at the intestinal epithelial barrier in inflammatory bowel disease. Inflamm Bowel Dis 2008;14:1000-11.
Howard OM, Ben-Baruch A, Oppenheim JJ. Chemokines: Progress toward identifying molecular targets for therapeutic agents. Trends Biotechnol 1996;14:46-51.
Horuk R. Chemokine receptors. Cytokine Growth Factor Rev 2001;12:313-35.
Zlotnik A, Yoshie O. Chemokines: A new classification system and their role in immunity. Immunity 2000;12:121-7.
Cole KE, Strick CA, Paradis TJ, Ogborne KT, Loetscher M, Gladue RP, et al.
Interferon-inducible T cell alpha chemoattractant (I-TAC): A novel Non-ELR CXC chemokine with potent activity on activated T cells through selective high affinity binding to CXCR3. J Exp Med 1998;187:2009-21.
Bizzarri C, Beccari AR, Bertini R, Cavicchia MR, Giorgini S, Allegretti M. ELR+CXC chemokines and their receptors (CXC chemokine receptor 1 and CXC chemokine receptor 2) as new therapeutic targets. Pharmacol Ther 2006;112:139-49.
Wuyts A, Van Osselaer N, Haelens A, Samson I, Herdewijn P, Ben-Baruch A, et al.
Characterization of synthetic human granulocyte chemotactic protein 2: Usage of chemokine receptors CXCR1 and CXCR2 andin vivo
inflammatory properties. Biochemistry 1997;36:2716-23.
Wolf M, Delgado MB, Jones SA, Dewald B, Clark-Lewis I, Baggiolini M. Granulocyte chemotactic protein 2 acts via both IL-8 receptors, CXCR1 and CXCR2. Eur J Immunol 1998;28:164-70.
Romagnani S. Induction of TH1 and TH2 responses: A key role for the 'natural' immune response? Immunol Today 1992;13:379-81.
Balabanian K, Lagane B, Infantino S, Chow KY, Harriague J, Moepps B, et al.
The chemokine SDF-1/CXCL12 binds to and signals through the orphan receptor RDC1 in T lymphocytes. J Biol Chem 2005;280:35760-6.
Legler DF, Loetscher M, Roos RS, Clark-Lewis I, Baggiolini M, Moser B. B cell-attracting chemokine 1, a human CXC chemokine expressed in lymphoid tissues, selectively attracts B lymphocytes via BLR1/CXCR5. J Exp Med 1998;187:655-60.
Farooq SM, Stillie R, Svensson M, Svanborg C, Strieter RM, Stadnyk AW. Therapeutic effect of blocking CXCR2 on neutrophil recruitment and dextran sodium sulfate-induced colitis. J Pharmacol Exp Ther 2009;329:123-9.
Ranganathan P, Jayakumar C, Manicassamy S, Ramesh G. CXCR2 knockout mice are protected against DSS-colitis-induced acute kidney injury and inflammation. Am J Physiol Renal Physiol 2013;305:F1422-7.
Chami B, Yeung AW, van Vreden C, King NJ, Bao S. The role of CXCR3 in DSS-induced colitis. PLoS One 2014;9:e101622.
Mascolo N, Izzo AA, Autore G, Maiello FM, Di Carlo G, Capasso F. Acetic acid-induced colitis in normal and essential fatty acid deficient rats. J Pharmacol Exp Ther 1995;272:469-75.
Dodda D, Chhajed R, Mishra J. Protective effect of quercetin against acetic acid induced inflammatory bowel disease (IBD) like symptoms in rats: Possible morphological and biochemical alterations. Pharmacol Rep 2014;66:169-73.
Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C (T) method. Nat Protoc 2008;3:1101-8.
Russo RC, Garcia CC, Teixeira MM, Amaral FA. The CXCL8/IL-8 chemokine family and its receptors in inflammatory diseases. Expert Rev Clin Immunol 2014;10:593-619.
Murphy PM, Baggiolini M, Charo IF, Hébert CA, Horuk R, Matsushima K, et al.
International union of pharmacology. XXII. Nomenclature for chemokine receptors. Pharmacol Rev 2000;52:145-76.
Bachelerie F, Ben-Baruch A, Burkhardt AM, Combadiere C, Farber JM, Graham GJ, et al.
International union of basic and clinical pharmacology. [corrected]. LXXXIX. Update on the extended family of chemokine receptors and introducing a new nomenclature for atypical chemokine receptors. Pharmacol Rev 2014;66:1-79.
Stillie R, Farooq SM, Gordon JR, Stadnyk AW. The functional significance behind expressing two IL-8 receptor types on PMN. J Leukoc Biol 2009;86:529-43.
Fu W, Zhang Y, Zhang J, Chen WF. Cloning and characterization of mouse homolog of the CXC chemokine receptor CXCR1. Cytokine 2005;31:9-17.
Natsui M, Kawasaki K, Takizawa H, Hayashi SI, Matsuda Y, Sugimura K, et al.
Selective depletion of neutrophils by a monoclonal antibody, RP-3, suppresses dextran sulphate sodium-induced colitis in rats. J Gastroenterol Hepatol 1997;12:801-8.
Buanne P, Di Carlo E, Caputi L, Brandolini L, Mosca M, Cattani F, et al.
Crucial pathophysiological role of CXCR2 in experimental ulcerative colitis in mice. J Leukoc Biol 2007;82:1239-46.
Ajuebor MN, Swain MG. Role of chemokines and chemokine receptors in the gastrointestinal tract. Immunology 2002;105:137-43.
Werner L, Elad H, Brazowski E, Tulchinsky H, Vigodman S, Kopylov U, et al.
Reciprocal regulation of CXCR4 and CXCR7 in intestinal mucosal homeostasis and inflammatory bowel disease. J Leukoc Biol 2011;90:583-90.
Mandai Y, Takahashi D, Hase K, Obata Y, Furusawa Y, Ebisawa M, et al.
Distinct roles for CXCR6(+) and CXCR6(-) CD4(+) T cells in the pathogenesis of chronic colitis. PLoS One 2013;8:e65488.
[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5]