Users Online: 1015
Home Print this page Email this page
Home About us Editorial board Search Browse articles Submit article Ahead of Print Instructions Subscribe Contacts Login 
Year : 2019  |  Volume : 8  |  Issue : 1  |  Page : 40

Microsatellite Marker Analysis for Laboratory Mice Profiling

1 Department of Nanobiotechnology, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran
2 Department of Quality Control, Pasteur Institute of Iran, Tehran, Iran
3 Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran
4 Department of Molecular Medicine, Pasteur Institute of Iran, Tehran, Iran

Correspondence Address:
Dr. Ramin Farhoudi
Department of Quality Control, Pasteur Institute of Iran, Tehran
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/abr.abr_53_19

Rights and Permissions

Background: The key point in the production procedure of inbred animals is checking the genetic purity. Skin grafting and coat color test are used traditionally to prove genetic purity, but they have some disadvantages. Recent advances in DNA profiling have enabled scientists to check easily the genetic purity of laboratory animals. In the current study, a set of microsatellite markers was designed to check the purity of inbred laboratory mice. Materials and Methods: Twenty microsatellites located on 20 chromosomes were employed to create a distinctive genetic profile for parentage analysis. Each individual primer was designed based on distinguishable colors and separable sizes. Results: Twenty specific microsatellite markers were used in the polymerase chain reaction mixture to identify inbred BALB/cJ strains. Our results confirmed that the designed microsatellites are excellent genetic markers for testing inbred BALB/cJ strain in laboratories. Conclusion: Our study showed that genetic profiling using microsatellite markers allows us to detect the genetic differences of laboratory mouse species in quality control tests and validation steps.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded151    
    Comments [Add]    

Recommend this journal