Nocardia Isolation of Soil
Mehdi Fatahi Bafghi1, Seyyed Saeed Eshraghi2
1 Department of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2 Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
|Date of Web Publication||31-Jan-2017|
Dr. Mehdi Fatahi Bafghi
Department of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd
Source of Support: None, Conflict of Interest: None
|How to cite this article:|
Bafghi MF, Eshraghi SS. Nocardia Isolation of Soil. Adv Biomed Res 2017;6:1
We read with interest article that published (Adv Biomed Res 2014; 3:151) by Faghri J et al. entitled *comparison of three phenotypic and deoxyribonucleic acid extraction methods for isolation and Identification of Nocardia spp.* however, authors and researchers should be noted that:
- According to the literature, Nocardia isolation of soil sample used of paraffin baiting technique, paraffin agar, humic acid-vitamin agar, sucrose-gradient centrifugation, conventional media with antibacterial and antifungal agents and diagnostic sensitivity test agar. Insome literature, paraffin baiting technique is the best method for Nocardia isolation of soil and clinical samples , but in this study, authors were reported is inappropriate that they did not optimize presumably
- Based on scientific evidence, growth in lysozyme broth is important for the genus Nocardia identification  but in this study, used of Gram-stain and partially acid-fast characteristics. Hence we should mention that Tsukamurella spp., Gordonia spp. and Rhodococcus spp. are partially acid-fast and Gram-positive ,,
- Authors used of slip-buried method with streptomycin/chloramphenicol for Nocardia isolation while many species of Nocardia are sensitive to chloramphenicol, so streptomycin is used for the treatment of Nocardia infections 
- In this article that describes DNA extraction methods from Nocardia, it needs revision based on the following comments:
- Authors mentioned that cetyltrimethylammonium bromide method is not satisfactory for DNA extraction while in some literature this method is suitable for DNA extraction of Nocardia and Mycobacterium
- Authors did not describe the amount of DNA recovered by the described method of extraction. This can be described in terms of the average µg of DNA per amount of cells and should include the range of DNA yield
- Authors indicated that extracted DNA was visualized on a 1% gel to assess quality and purity, but did not indicate the amount of DNA run on the gel. In addition, no additional method of assessment of DNA purity was used such as the standard ultraviolet spectrophotometry method, which generates A260/A280 ratios.
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| References|| |
Yamamura H, Hayakawa M, Iimura Y. Application of sucrose-gradient centrifugation for selective isolation of Nocardia
spp. from soil. J Appl Microbiol 2003;95:677-85.
Singh M, Sandhu RS, Randhawa HS. Comparison of paraffin baiting and conventional culture techniques for isolation of Nocardia asteroides
from sputum. J Clin Microbiol 1987;25:176-7.
Habibnia S, Heidarieh P, Pourmand M, Fatahi M, Eshraghi S. Comparison of paraffin bait, humic acid Vitamin B agar and paraffin agar methods to isolate Nocardia
from soil. Med Lab J 2014;7:29-36.
Goodfellow M. Characterisation of Mycobacterium
and related taxa. Ann Soc Belg Med Trop 1973;53:287-98.
Liu CY, Lai CC, Lee MR, Lee YC, Huang YT, Liao CH, et al.
Clinical characteristics of infections caused by Tsukamurella
spp. and antimicrobial susceptibilities of the isolates. Int J Antimicrob Agents 2011;38:534-7.
Emmons W, Reichwein B, Winslow DL. Rhodococcus equi
infection in the patient with AIDS: Literature review and report of an unusual case. Rev Infect Dis 1991;13:91-6.
Pham AS, Dé I, Rolston KV, Tarrand JJ, Han XY. Catheter-related bacteremia caused by the nocardioform actinomycete Gordonia
terrae. Clin Infect Dis 2003;36:524-7.
Garrett MA, Holmes HT, Nolte FS. Selective buffered charcoal-yeast extract medium for isolation of Nocardiae
from mixed cultures. J Clin Microbiol 1992;30:1891-2.
Dewsnup DH, Wright DN.In vitro
susceptibility of Nocardia asteroides
to 25 antimicrobial agents. Antimicrob Agents Chemother 1984;25:165-7.
Vera-Cabrera L, Johnson WM, Welsh O, Resendiz-Uresti FL, Salinas-Carmona MC. Distribution of a Nocardia brasiliensis
catalase gene fragment in members of the genera Nocardia
, and Rhodococcus
. J Clin Microbiol 1999;37:1971-6.