Users Online: 718
Home Print this page Email this page
Home About us Editorial board Search Browse articles Submit article Ahead of Print Instructions Subscribe Contacts Login 
ORIGINAL ARTICLE
Year : 2017  |  Volume : 6  |  Issue : 1  |  Page : 17

Treatment Outcome of the Drug-resistant Zoonotic Cutaneous Leishmaniasis by Glucantime


1 Department of Parasitology, School of Medicine, Isfahan University of Medical Sciences and Health Services, Isfahan, Iran
2 Department of Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3 Department of Anatomical Sciences and Molecular Biology, Medical School, Isfahan University of Medical Sciences and Health Services, Isfahan, Iran
4 Department of Parasitology, School of Medicine, Isfahan University of Medical Sciences and Health Services, Isfahan; Department of Parasitology, Skin Disease and Leishmaniasis Research Center, School of Medicine, Isfahan University of Medical Sciences and Health Services, Isfahan, Iran

Correspondence Address:
Dr. Seyed Hossain Hejazi
Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences and Health Services, Isfahan
Iran
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2277-9175.201329

Rights and Permissions

Background: Resistance of Leishmania species to antimonial drugs has increased. Hence, in the present study Leishmania major isolates were collected from patients with resistance phenotype and the presence/absence of resistance to Glucantime was investigated. Materials and Methods: Samples were taken from 10 cutaneous leishmaniasis (CL) patients who had not responded to chemotherapy with Glucantime. Nested polymerase chain reaction (PCR) was performed to identify the isolated species. Stationary phase promastigotes were added to the grown, adhesive J774 macrophages. Values obtained from standard strain were compared with the test cultures after exposure to the medicine. In vivo, the effects of Glucantime were assessed by comparing the sizes and the parasite burden of the lesions on mouse model. Results: The results of amplified band on agarose gel demonstrated all samples were L. major. After exposure to medicine, a reduction of intracellular amastigotes to half was detected. In vivo, the parasite was eliminated in 90% of mice with lesions caused by both isolates of patients and standard L. major, and their lesions became smaller significantly. Conclusion: Pentavalent antimonial (SbV) salts are the main component of chemotherapy against leishmaniasis. However, the medicine has been found ineffective. In the present study, isolates from patients with no response to treatment had no significant difference from the standard L. major strain (as the sensitive strain). Therefore, in patients with resistance phenotype to Glucantime, the parasites did not actually have intrinsic resistance, i.e., environmental and host factors prevented the successful treatment of the disease.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1270    
    Printed26    
    Emailed0    
    PDF Downloaded174    
    Comments [Add]    
    Cited by others 1    

Recommend this journal