Yoga intervention on blood NO in female migraineurs
Mahsa Zamani Boroujeni1, Seyed Mohamad Marandi2, Fahimeh Esfarjani2, Mina Sattar2, Vahid Shaygannejad3, Shaghayegh Haghjooy Javanmard1
1 Department of Physiology, Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
2 Department of Exercise Physiology, University of Isfahan, Isfahan, Iran
3 Department of Neurology, Isfahan University of Medical Sciences, Isfahan, Iran
|Date of Submission||13-Mar-2014|
|Date of Acceptance||25-Jul-2014|
|Date of Web Publication||31-Dec-2015|
Shaghayegh Haghjooy Javanmard
Assistant Professor of Physiology, Physiology Research Center, Isfahan University of Medical Sciences, Hezar Jerib Avenue, Isfahan
Source of Support: None, Conflict of Interest: None
Background: The current survey investigates the effect of 12 weeks yoga training on headache frequency, severity, duration and blood nitric oxide levels as well as headache impacts on female migraineurs' lives.
Materials and Methods: Thirty-two female patients with migraine took part and were randomly divided into two groups. The control group (n = 14) received medication and the yoga group (n = 18) participated in 12 weeks yoga training in addition to receiving the same medication as that of the control group. Frequency and duration of headache were assessed by a questionnaire. Visual Analogue Scale was used to measure the severity of headache, and the metabolite of NO also was measured by Griess reaction. Headache Impact Test ( HIT-6) was also used to assess the impact of headache on patients' lives. Data were analyzed by t-test mean variance.
Results: After 3 months intervention, in the yoga group, there was a significant reduction in the impact of headache on patients' lives, headache frequency, and severity and a non-significant reduction in headache duration in the yoga group. There was no significant difference in the plasma levels of NO between yoga and control groups before and after the study.
Conclusion: Based on the results, yoga could be recommended as a complementary method to migraine patients.
Keywords: Asana, headache impact, migraine, NO, pranayama, shavasana
|How to cite this article:|
Boroujeni MZ, Marandi SM, Esfarjani F, Sattar M, Shaygannejad V, Javanmard SH. Yoga intervention on blood NO in female migraineurs. Adv Biomed Res 2015;4:259
|How to cite this URL:|
Boroujeni MZ, Marandi SM, Esfarjani F, Sattar M, Shaygannejad V, Javanmard SH. Yoga intervention on blood NO in female migraineurs. Adv Biomed Res [serial online] 2015 [cited 2020 Jan 28];4:259. Available from: http://www.advbiores.net/text.asp?2015/4/1/259/172995
| Introduction|| |
Migraine headaches are often a debilitating condition which affects millions of people throughout the world. Migraine is a serious neurological disorder which is unilateral, repeated alternatively, and occurs usually along with vomiting, nausea, photophobia, and phonophobia.  Although the prevalence of migraine may be slightly different in different societies, it is strikingly higher in females than in males and its ratio is changing from 2:1 to 3:1, especially between the ages of 25 and 55 years.  Studies show that migraine can not only be a risk factor of cardiovascular diseases and ischemic stroke, but it also has a relationship with brain structural injuries and lesions like those of brain circulation which lead to brain stroke. , The majority of the studies show a kind of depression in women migraine sufferers.  Food intolerance and allergies,  structural problems,  hormonal imbalances,  low blood sugar,  hard work,  brain tumors,  cerebrovascular hypoxia caused by reduced blood supply to the brain vessels,  depression,  high blood pressure,  neck trauma,  and many other unmentioned factors can cause migraine or make it more severe. Based on genetic evidences, it is strongly believed that changes in blood vessels, disruption in endocrine system, and some micro metabolism can create a dysfunction in neurovascular function and cause cortex depression. , There are important mediators like the neuropeptides substance P, calcitonin gene-related peptide (CGRP), and vasoactive intestinal polypeptide (VIP), which lead to a severe impairment in cerebral cortex function, vessel expansion, and cause migraine or primary headaches.  At that time, an electrical current moves to the frontal lobe at a certain speed and causes headache. 
Nitric oxide (NO) is an endothelium-derived relaxing factor (EDRF)-like substance which plays a key role in regulating brain metabolism and cerebral circulation, and vascular smooth muscle function.  It is one of the responsible factors for cerebral blood flow modulation in response to changes in oxygen gas and carbon dioxide; hence, it is not unlikely that impairment in NO production causes several pathological conditions of brain such as migraine, Alzheimer's and Huntington's, and even inflammation.  Repeated exercise helps NO to be kept in higher levels in the blood between exercise sessions. However, in apparent contrast, some surveys have suggested that some kinds of exercise may intensify migraine attacks due to excess NO production.  Yoga, as a Complementary and Alternative Medicine (CAM), is a popular alternative form of mind-body therapy. Due to higher prevalence of migraine in women and the popularity of yoga among them, in this survey, the effects of this kind of exercise on the frequency, severity, and duration of headache, the impact of headache on patient's life, and the blood NO levels of female patients with migraine have been studied.
Study design and setting
This study was a randomized, controlled trial conducted at the Physiology Research Center, University of Medical Sciences, Isfahan, Iran, from April to June 2012. The Ethical Committee of Isfahan University of Medical Sciences approved the project and the protocol. Filled out the consent form before baseline assessment and randomization.
| Materials And Methods|| |
Patients were selected from a Neurological Institute under the supervision of a neurologist. All potential subjects were informed that we were conducting a study of migraine treatment that was intended to reduce its negative effects on their personal, family, and social lives. Evaluations (with diagnosis) were performed by neurologists, and detailed case histories were taken by trained interviewers. The criteria for selection of the subjects among the other migraineurs were according to the definition of the International Headache Society (IHS).  All the patients were in menstrual age and have not had any experience of yoga training before. They also were under a same pharmacological treatment and did not have any other exercise during the treatment period.
Eighty-five patients agreed to participate in the program. Forty-three patients were excluded. Seventeen of them were excluded as they were receiving different medical treatments. Seven migraineurs were crossed out due to coexisting diseases and nineteen patients did not meet the inclusion criteria. The rest of the migraine patients were accidently divided into two groups (yoga group and control group). A computer-generated random number list allocated patients to either case or control group. In the control group, four patients left the intervention because of worsening of their symptoms, two refused to participate in blood test, and one left the program because our exercise time overlapped with her work schedule. In the experimental group, one patient refused to continue because of drugs' side effects and two patients left the treatment due to unknown reason. Thirty-two patients were finally analyzed. The steps are shown in [Figure 1].
Patients were informed of the details of the treatment plan. The initial examination and medication were performed by a neurologist who confirmed the diagnosis of migraine. Personal information, family headache history, frequency and duration of headache, and medication were recorded.
Severity of headache
Visual Analogue Scale (VAS) was also used to measure the severity of headache pain. The VAS has been shown to be reliable and valid for measuring pain. It is also applied in different clinical surveys including migraine, low back pain, colon cancer, etc. ,,, Each subject expresses her headache intensity on a 100-mm VAS that ranges from no pain (0) to very severe pain. 
The headache impact test
The headache impact test (HIT-6) was developed to measure a wide range of factors contributing to the burden of headache, and it has shown utility for producing quantitative and pertinent information on the impact of headache. The HIT-6 consists of six items: Pain, social functioning, role functioning, vitality, cognitive functioning, and psychological distress. The patient answers each of the six related questions with one of the following five responses: "Never", "rarely", "sometimes", "very often", and "always". These responses are summed to produce a total HIT-6 score that ranges from 36 to 78, where a higher score indicates a greater impact of headache on the daily life of the respondent. Scores can be interpreted using four groupings that demonstrate the severity of impact of headache on the patient's life. Extensive testing has shown the HIT-6 to be highly reliable and internally consistent, and it has been translated into various languages. The HIT-6 also exhibits excellent accessibility and ease of use. Although HIT-6 is a very promising instrument, little data exists for comparing HIT-6 scores with the real amount of disability, which would help in interpreting the results when creating a management plan. 
Serum nitrite measurement
The level of serum nitrite (stable NO metabolite) was measured using a colorimetric assay kit (R&D Systems, Minneapolis, MN, USA) that involves the Griess reaction. Briefly, serum was added into wells (96-well enzymatic assay plate). Sulfanilamide solution was added to all experimental samples, and after incubation, N-1-naphtylethylenediamine dihydrochloride solution was added. Then, the absorbance was measured using a microreader at 540 nm wavelength. The sample's nitrite concentration was determined on comparing it to a nitrite standard reference curve. The detection limit was 0.25 μM nitrite.
Serum NOx and nitrate measurement
For measuring the total nitrite (NOx) concentration in serum samples, total NO/nitrite/nitrate assay kit (R & D Systems) was used which is based on the enzymatic conversion of nitrate to nitrite by nitrate reductase. The reaction is followed by colorimetric detection of nitrite as an azo dye product of the Griess reaction. Briefly, serum samples were added into wells (96-well enzymatic assay plate). Then equal volume of NADH and dilute nitrate reductase were added to all wells. The samples were incubated for 30 min at 37°C. At the end of this step, nitrate in the samples gets reduced to nitrite. Then the concentration of nitrite in the samples was measured as mentioned above. For determining the nitrate concentration in the sample, the endogenous nitrite concentration was subtracted from the NOx concentration. 
Patients were randomly divided into two groups (yoga and control groups). The control group received only medication for 12 weeks and the yoga group was placed in a yoga training program that consisted of three sessions per week (each session 75 min) in addition to the same medical treatment. During 3 months, the control group was under the supervision of a neurologist, medically and mentally. Yoga program was chosen from Hatha yoga Pradipika. It included asanas (yoga movements), pranayama (breathing exercises), and shavasanas (relaxation). Asanas largely dealt with the positions related to head and neck, although lower extremity, arms, and shoulder exercises were also involved. Eye-related exercises, pavanmoktasana, pre-pranayama and pranayama, positions in lying, sitting, and standing status, palming, Neti exercises, and Shavasana were the kinds of exercises which were done under the supervision of a trainer. Surya namaskar was another part of the program, which includes 12 positions itself, and it is mainly used for warm up, stretch, strength, and increasing flexibility. The protocol details are given in [Table 1].
Blood test was performed for all patients in the physiology research center at Isfahan Faculty of Medicine between 8 a.m. and 10 a.m. in a fasting condition. In the first session of exercises, patients were asked to mark their headache frequency during these 3 months in a table to which they were given.
All statistical analyses were performed using SPSS for Windows version 16.0. All data were normally distributed and presented as mean ± SD. Independent t-test was used to compare the mean differences between groups before and after training. Differences of P ≤ 0.01 were considered significant for all statistical analyses.
| Results|| |
The mean age was 35.4 ± 7.9 years in the exercise group and 34.9 ± 8.37 years in the control group. The body mass index (BMI) in the yoga group was 66.8 ± 4.47 and in the control group was 63.8 ± 6.19. When comparing yoga and control groups after 12 weeks, the results showed a reduction in headache severity, frequency, and headache impact on patients' lives after the intervention in the yoga group [Figure 2], [Figure 3], [Figure 4]; however, the changes in the control group were not significant. Duration of headache also reduced after 12 weeks in the yoga group, but the results were not significant [Figure 5]. A non-significant increase was seen in blood NO level in both groups after treatment [Figure 6]. The mean, standard deviation, and independent t-test and other results which are related to this study are shown in [Table 2].
|Figure 2: Severity of headache before and after yoga training between yoga and control groups|
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|Figure 3: Frequency of headache before and after yoga training between yoga and control groups|
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|Figure 4: Impact of headache before and after yoga training between yoga and control groups|
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|Figure 5: During of Headache before and after yoga training between yoga and control groups|
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|Figure 6: Nitric Oxide before and after yoga training between yoga|
and control groups
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|Table 2: Mean ± SD of the results before and after intervention in the study groups|
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| Discussion|| |
The aim of this research was to investigate the effectiveness of yoga-based intervention on migraine headache and endothelial dysfunction. Although the reduction in duration of headache was not significant in this study, yoga was found to have a beneficial effect on various migraine parameters (frequency, intensity, duration of attack, medication score, and the nature of the pain). , One contemporary theory of how migraines occur states that the arteries that feed the brain narrow temporarily due to a variety of factors, with stress and muscular tension being the two common ones. For a migraine sufferer, there is a sudden shift in the blood vessels and they abruptly dilate, increasing the blood flow to the head. This occurrence results in the severe pain of the migraine episode. If patients can somehow keep their body's nervous system more relaxed daily, the initial narrowing of the blood vessels that predisposes someone to a migraine might be eliminated and the chance of the migraine is minimized. ,,
Many surveys and experiments have shown that aerobic exercise significantly leads to cardiovascular fitness improvement, regulates vessel tone regulation, and decreases migraine attacks. 
Yoga also affect psychological parameters like anxiety and depression which are the causes of the headache impact on the life of migraine sufferers.  Yogic breathing is useful for balancing the autonomic nervous system and has a special influence on psychological and stress-related disorders.  Certain mechanisms which describe a state of calm alertness include increased parasympathetic drive, calming of stress response systems, neuroendocrine release of hormones, and thalamic generators. 
If done correctly, yoga can significantly release tensions accumulated around the areas of pain (forehead, temples, neck, and shoulders) and also loosen the tight muscles, especially in the back and neck. Since tight muscles can trigger headaches, yoga could potentially assist. 
Nasal water cleansing (Kriya-Jalaneti) which is followed by forced exhalation (Kapalbhanti) goes beyond stimulating and regulating the nerve tone, glands, and organs of the entire nasal and cranial area, including the eyes, sinuses, ears, and cranium. 
During pranayama, baseline O 2 consumption increases which could be due to the increased sympathetic stimulation of the adrenal medulla. Since hypoxia is one of the risk factors of migraine, increasing the O 2 consumption by pranayama can be a solution.  We suggested that the NO was also increased in our survey, although the levels were not significant. Vascular endothelial function is vital for maintenance of health of vessel walls and for vasomotor control. Regular exercises have been demonstrated to regulate the expression of the endothelial nitric oxide synthase (NOS) gene in the vascular tissue. ,, On the other hand, it is also known that relatively intense exercises can provoke migraine headache or worsen it. ,, Some investigators have opined that this might be linked to an acute rise in the blood NO level. Some other studies demonstrated increased l-arginine/NO pathway activity in platelets from patients with migraine, and this influence is particularly noticeable during the attacks.  The non-significant levels of blood NO in our study may be due to the low intensity of exercises compared with aerobic exercises. So, it is recommended to make a comparative survey between the effects of aerobic exercises and yoga training.
On the basis of above remarks, the mind-body activities in yoga enhance vascular health and promote a feeling of well-being. , In addition, yoga asanas also improve the body's physical and mental fitness which may help control blood pressure, respiration, and heart and metabolic rates.  On the other hand, yoga training helps patients to get over stress and anxiety, the factors which are known to intensify migraine setup, its severity, and also the frequency.  Yogic theory and practice lead to increased self-knowledge. This knowledge is not merely that of the practical kind related to techniques, but especially of a spiritual sort pertaining to grasping something about the nature of the self at rest.
| Conclusion|| |
It is too early to recommend yoga for treatment or prevention of migraines. However, there is some evidence showing that taking up yoga could enhance overall health. It controls the negative impacts of migraine and helps patients lead a healthy lifestyle, be more effective and live happier. It also eliminates the modifiable risk factors for the other diseases. 
| Acknowledgment|| |
This study was sponsored by Isfahan University of Medical Sciences and Department of Exercise Physiology, University of Isfahan.
| References|| |
Mirzaei GM. A review of common migraine in girls high school and related fall in education in Shahrekord region. J Shahrekord Univ Med Sci 2004;5:55-62.
Buse DC, Loder EW, Gorman JA, Stewart WF, Reed ML, Fanning KM, et al
. Sex differences in the prevalence, symptoms, and associated features of migraine, probable migraine and other severe headache: Results of American migraine prevalence and prevention (AMPP) study. Headache 2013;53:1278-99.
Vanmolkot FH, Van Bortel LM, de Hoon JN. Altered arterial function in migraine of recent onset. Neurology 2007;68:1563-70.
Rist PM, Diener HC, Kurth T, Schürks M. Migraine, migraine aura, and cervical artery dissection: A systematic review and meta-analysis. Cephalalgia 2011;31:886-96.
Rist PM, Schürks M, Buring JE, Kurth T. Migraine, headache, and the risk of depression: Prospective cohort study. Cephalalgia 2013;33:1017-25.
Savi L, Rainero I, Valfrè W, Gentile S, Lo Giudice R, Pinessi L. Food and headache attacks. A comparison of patients with migraine and tension-type headache. Panminerva Med 2002;44:27-31.
Singman EL, Matta NS, Silbert DI. Case report: Migraine caused by abnormal head posture in a patient with ptosis. Am Orthopt J 2010;60:101-2.
Hershey AD. Menstrual migraine: How early can it start? Headache 2009;49:348-9.
Hanington E. Diet and migraine. J Hum Nutr 1980;34:175-80.
Diamond S, Freitag FG, Solomon GD, Millstein E. Migraine headache. Working for the best outcome. Postgrad Med 1987;81:174-6, 179-83.
Eszenyi-Halasy M, Lehoczky T. Migraine and brain tumor. Acta Med Acad Sci Hung 1953;4:93-104.
Amery WK. Migraine and cerebral hypoxia: A hypothesis with pharmacotheraputic implications. Cephalalgia 1985;5 (Suppl 2):131-3.
Robbins MS, Bronheim R, Lipton RB, Grosberg BM, Vollbracht S, Sheftell FD, et al
. Depression and anxiety in episodic and chronic cluster headache: A pilot study. Headache 2012;52:600-11.
Tronvik E, Zwart JA, Hagen K, Dyb G, Holmen TL, Stovner LJ. Association between blood pressure measures and recurrent headache in adolescents: Cross-sectional data from the HUNT- youth study. J Headache Pain 2011;12:347-53.
Láinez MJ, Piera A, Bono G. Headache attributed to head or neck trauma. Handb Clin Neurol 2010;97:501-16.
Lauritzen M. Cortical spreading depression in migraine. Cephalalgia 2001;21:757-60.
Schoenen J. The pathophysiology of migraine: A review based on the literature and on personal contributions. Funct Neurol 1998;13:7-15.
Messlinger K, Fischer MJ, Lennerz JK. Neuropeptid effects in trigeminal system: Pathophysiology and clinical relevance in migraine. Keio J Med 2011;60:82-9.
Shimizu T, Suzuki N. Perspective of pathophysiology and treatment of migraine. Brain Nerve 2012;64:59-64.
Green DJ, Maiorana A, O'Driscoll G, Tailor R. Effect of exercise training on endothelium- derived nitric oxide function in humans. J Physiol 2004;561:1-25.
Toda N, Ayajiki K, Okamura T. Cerebral flow regulation by nitric oxide: Recent advances. Pharmacol Rev 2009;61:62-97.
Narin SO, Pinar L, Erbas D, Oztürk V, Idiman F. The effects of exercise and exercise-related changes in blood nitric oxide level on migraine headache. Clin Rehabil 2003;17:624-30.
Classification and diagnostic criteria for headache disorders, cranial neuralgias and facial pain. Headache Classification Committee of the International Headache Society. Cephalalgia 1988;8 (Suppl 7):1-96.
Zengin MO, Elmas Z, Cinar E, Kucukerdonmez C. Choroidal thickness changes in patients with migraine. Acta Neurol Belg 2014. [Epub ahead of print].
Turkcuer I, Serinken M, Eken C, Yilmaz A, Akdag Ö, Uyan E, et al
. Intravenous paracetamol versus dexketoprofen in acute migraine attack in the emergency department: A randomised clinical trial. Emerg Med J 2014;31:182-5.
Pirbudak L, Sevinç A, Maralcan G, Kýlýç E. Pain management with intrathecal clonidine in a colon cancer patient with opioid hyperalgesia: Case presentation. Agri 2014;26:93-6.
Yamada K, Suzuki A, Takahashi S, Yasuda H, Koike T, Nakamura H. Severe low back pain in patients with rheumatoid arthritis is associated with disease activity score but not with radiological findings on plain X-rays. Mod Rheumatol 2014;19:1-6.
Pai S, Ghezzi EM, Ship JA. Development of visual analogue scale questionnaire for subjective assessment of salivary dysfunction. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2001;91:311-6.
Shin HE, Park JW, Kim YI, Lee KS. Headache Impact Test-6 (HIT-6) scores for migraine patients: Their relation to disability as measured from a headache diary. J clin Neurol. 2008;4:158-63.
Haghjooy Javanmard S, Nematbakhsh M, Monajemi A. L-arginine supplementation influenced nitrite but not nitrate and total nitrite in rabbit model of hypercholesterolemia. Iran Biomed J 2008;12:179-84.
Sharma N, Singhal S, Singh AP, Sharma CM. Effectiveness of integrated yoga therapy in treatment of chronic migraine: Randomized controlled trial. J Headache Pain 2013;14 (Suppl 1):116.
John PJ, Sharma N, Sharma CM, Kankane A. Effectiveness of yoga therapy in the treatment of migraine without aura: A randomized controlled trial. Headache 2007;47:654-61.
http://www.yogajournal.com/practice/974. [Last accessed on 2013 Aug 12]
Chillot R. Stop headache… like magic. Prevention 1998;50:98-108.
Darling M. The use of exercise as a method of aborting migraine. Headache 1991;31:616-8.
Swain RA, Kaplan B. Diagnosis, prophylaxis and treatment of headaches in the athlete. South Med J 1997;90:878-88.
Wells RE, Bertisch SM, Buettner C, Phillips RS, McCarthy EP. Complementary and alternative medicine use among adults with migraines/severe headaches. Headache 2011;51:1087-97.
Narin SO, Pinar L, Erbas D, Oztürk V, Idiman F, The effects of exercise and exercise-related changes in blood nitric oxide level on migraine headache. Clin Rehabil 2003;17:624-30.
Jungersten L, Ambring A, Wall B, Wennmalm A. Both physical fitness and acute exercise regulate nitric oxide formation in healthy humans. J Appl Physiol (1985) 1997;82:760-4.
Available from: http://www.healthandyoga.com/html/research_papers/pp.aspx. [Last accessed on 2013 Mar 10].
Bradley WG, Daroff RB, Fenichel GM, Marsden CD. Neurology in Clinical Practice: Principles of Diagnosis and Management. 2 nd
edition, Volume 2. Oxford: Butterworth Heinemann; 1996. p. 1695-701.
Varin R, Mulder P, Richard V, Tamion F, Devaux C, Henry JP, et al
. Exercise improves flow-mediated vasodilatation of skeletal muscle arteries in rats with chronic heart failure. Role of nitric oxide, prostanoids, and oxidant stress. Circulation 1999;99:2951-7.
Diamond S. Managing migraines in active people. Phys Sportsmed 1996;24:41-53.
Thomsen LL, Olesen J. Nitric oxide theory of migraine. Clin Neurosci 1998;5:28-33.
Indo T, Takahashi A. Swimmer's migraine. Headache 1990;30:485-7.
Sarris J, Moylan S, Camfield DA, Pase MP, Mischoulon D, Berk M, et al
. Complementary medicine, exercise, meditation, diet, and lifestyle modification for anxiety disorders: A review current of evidence. Evid Based Complement Alternat Med 2012;2012:809653.
Manchanda SC, Narang R. Yoga for coronary artery disease. Indian Heart J 1998;50:227-8.
Murugesan R, Govindarajulu N, Bera TK. Effect of selected yogic practices on the management of hypertension. Indian J Physiol Pharmacol 2000;44:207-10.
Li AW, Goldsmith CA. The effects of yoga on anxiety and stress. Altern Med Rev 2012;17:21-35.
[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6]
[Table 1], [Table 2]