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ORIGINAL ARTICLE
Year : 2015  |  Volume : 4  |  Issue : 1  |  Page : 201

In silico prediction of B- and T- cell epitope on Lassa virus proteins for peptide based subunit vaccine design


1 Department of Biotechnology, Madhav Institute of Technology and Science, Gwalior, Madhya Pradesh, India
2 Department of Biotechnology, Faculty of Engineering and Technology, Rama University, Kanpur, Uttar Pradesh, India

Correspondence Address:
Sitansu Kumar Verma
Department of Biotechnology, Madhav Insti tute of Technology and Science, Gwalior, Madhya Pradesh - 474 005
India
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Source of Support: Nil, Conflict of Interest: None declared.


DOI: 10.4103/2277-9175.166137

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Background: Lassa fever is a severe, often-fatal and one of the most virulent disease in primates. However, the mechanism of escape of virus from the T-cell mediated immune response of the host cell is not explained in any studies yet. In our studies we had aimed to predict B- and T- cell epitope of Lassa virus protein, for impaling the futuristic approach of developing preventive measures against this disease, further we can also study its presumed viral- host mechanism. Materials and Methods: Peptide based subunit vaccine was developed from all four protein against Lassa virus. We adopted sequence, 3D structure and fold level in silico analysis to predict B-cell and T-cell epitopes. The 3-D structure was determined for all protein by homology modeling and the modeled structure validated. Results: One T-cell epitope from Glycoprotein (WDCIMTSYQ) and one from Nucleoprotein (WPYIASRTS) binds to maximum no of MHC class I and MHC class II alleles. They also specially bind to HLA alleles namely, A*0201, A*2705, DRB*0101 and DRB*0401. Conclusions: Taken together, the results indicate the Glycoprotein and nucleoprotein are most suitable vaccine candidates against Lassa virus.


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