Users Online: 440
Home Print this page Email this page
Home About us Editorial board Search Browse articles Submit article Ahead of Print Instructions Subscribe Contacts Login 


 
Previous article Browse articles Next article 
ORIGINAL ARTICLE
Adv Biomed Res 2014,  3:106

Macroscopic effect of blue light cure on wound healing in NMRI mice NMRI


1 Skin Diseases and Leishmaniasis Research Center, Department of Dermatology, Isfahan University of Medical Sciences, Isfahan; Skin and Stem Cell Research Center, Department of Dermatology, Tehran University of Medical Sciences, Tehran, Iran
2 Department of Radiology and Medical Physics, Tehran University of Medical Sciences, Tehran, Iran
3 Department of Dental Pathology, Islamic Azad University of Khorasgan, Isfahan, Iran
4 Department of Physiology and Biophysics, Islamic Azad University of Tehran, Tehran, Iran
5 Skin and Stem Cell Research Center, Department of Dermatology, Tehran University of Medical Sciences, Tehran; Skin Diseases and Leishmaniasis Research Center, Department of Dermatology, Isfahan University of Medical Sciences, Isfahan, Iran
6 Skin Diseases and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Date of Submission05-Mar-2013
Date of Acceptance12-Mar-2014
Date of Web Publication31-Mar-2014

Correspondence Address:
Mohammad Ali Nilforoushzadeh
Skin and Stem Cell Research Center, Department of Dermatology, Tehran University of Medical Sciences, Tehran
Iran
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2277-9175.129699

Rights and Permissions
  Abstract 

Background: Wound healing is a complex process and has been an ongoing challenge all over the world. Some studies have suggested light cure as a modality to accelerate wound repair. It can induce fibroblast proliferation, increase collagen synthesis and activate cellular processes involved in expression of procollagen type I and III mRNA. This study was designed to assess the macroscopic effect of halogen dental curing blue light on full-thickness open wound healing in NMRI mice.
Materials and Methods: Forty male NMRI mice were divided into control and treatment groups. A full-thickness wound of 6 mm in diameter was induced on the lower back of all mice under general anesthesia and sterile conditions. The mice of the treatment group received a 5-min exposure of halogen light Coltolux II (QHL), 420-500 nm, daily for 7 days. The diameter of the wound was measured in both the treatment and the control groups every second day up to Day 14. Data were analyzed by SPSS version 12 software using Student's t-test. A significance level of P ≤ 0.05 was considered for each comparison.
Results: There was a significant difference in wound diameter between the control and the treatment groups at all measurements after Day 3 (P ≤ 0/05).
Conclusion: The results of this study suggest improvement of full-thickness wound healing by daily irradiation of halogen dental curing blue light of 420-500 nm for 7 days.

Keywords: Light cure, NMRI mice, wound healing


How to cite this article:
Jaffary FA, Changizi V, Mardani H, Kakanezhadian P, Javadi FM, Nilforoushzadeh MA, Haftbaradaran E. Macroscopic effect of blue light cure on wound healing in NMRI mice NMRI. Adv Biomed Res 2014;3:106

How to cite this URL:
Jaffary FA, Changizi V, Mardani H, Kakanezhadian P, Javadi FM, Nilforoushzadeh MA, Haftbaradaran E. Macroscopic effect of blue light cure on wound healing in NMRI mice NMRI. Adv Biomed Res [serial online] 2014 [cited 2019 Oct 18];3:106. Available from: http://www.advbiores.net/text.asp?2014/3/1/106/129699


  Introduction Top


There are several studies on the effect of laser therapy and wound healing suggesting wound healing speed-up following different laser protocols while still more studies are needed to determine the optimum parameters for this situation. [1] The wound-healing process involves a series of complex responses that are affected by different factors like etiologic basis of the wound, type of the tissue and general systemic condition of the patients. [2] Besides pharmacotherapy, other modalities such as stem cell therapy, [3] hyperbaric oxygen, [4],[5] phototherapy and laser [6],[7],[8] have been suggested to improve wound healing. Whinfield and Aitkenhead have shown in their review that phototherapy may have positive effects in promoting wound healing in vitro and animal studies, but there was no established efficacy in clinical trials, and further studies are required to determine the role of phototherapy in wound care. [9]

Two meta-analyses of the efficacy of low-level laser in wound repair have revealed the efficacy of laser therapy for promoting wound healing [10],[11] and pain relief. [11] A subgroup analysis of data showed a significant effect on collagen synthesis, rate of healing, tensile strength, wound size, time to wound closure, tensile stress, number and rate of degranulation of mast cells and flap survival. [12],[13] Halogen dental cure emitting blue light of 420-500 nm is being used to cure dental composites for years. This is an innovative use of this device as an easy access affordable device for improvement of wound healing, and the following study was designed to test this idea.


  Materials and Methods Top


Forty male NMRI mice, weighing 20-25 g, were used for performing this experiment. Mice were provided from the Tehran Science and Research Azad University and all the experiments were performed in the Professor Torabinejad Research Center in Isfahan. This trial was approved by the ethical committee of the Tehran Science and Research Azad University on 14 September 2008. Animals were housed in cages on a 12 h:12 h light/dark cycle at 20-25°C with free access to food and water. After shaving the fur, a full-thickness circular wound of 6 mm in diameter was induced by a punch on the back of the mice (area above the tail) under ether anesthesia in all mice. All animals received an intramuscular injection of penicillin-gentamycin to prevent wound infection. Animals of the treatment group were subjected to 5-min blue light irradiation (Coltolux II [QHL], Coltene, Switzerland) from Days 1 to 7. Wound diameter was measured using a millimeter scaled paper on Days 1, 3, 5, 7, 10 and 13 after injury in both groups.

Data were analyzed by SPSS version 12 software using Student's t-test. A significance level of P ≤ 0.05 was considered for each comparison.


  Results Top


Data of wound diameter in the control and treatment groups is shown in [Table 1] and [Figure 1]. Except for Day 1, the wound diameter was significantly less in the treatment group compared with the control group at all measurements (P ≤ 0.01).
Figure 1: Line chart of the mean wound diameter at Days 1, 3, 5, 7, 10 and 13 based on the groups

Click here to view
Table 1: Comparison of the mean wound diameter at Days 1, 3, 5, 7, 10 and 13 in both the control and the treatment groups

Click here to view



  Discussion Top


This is the first study on using dental blue light cure for improvement of wound healing. The results of this study showed efficacy of 5-min 1-week irradiation by this blue light in improvement of full-thickness wound healing in NMRI mice. Considering more accessibility of this device for the patients compared with LED or other laser-emitting systems, it could be an attracting option for in-home use by patients with chronic ulcer.

Demidova-Rice et al. have reported significant improvement of wound healing in BALB/c and SKH1 hairless mice with 635 nm light. [14] There was no difference in the efficacy of the same wavelength from a lamp or He-Ne laser source. Da Silva 2010 reviewed studies on the use of laser therapy in the tissue repair process with a focus on the different lasers and applied parameters. [1] The closest wavelength to blue light dental cure is helium neon laser (He-Ne laser, 632.8 nm). Based on the available studies, several mechanisms could be suggested for improvement of wound healing by this laser. Significant increase in proliferation and differentiation of human osteoblasts, promoting migration, cell proliferation and viability of injured fibroblasts, and stimulating mitochondrial activity are reported in in vitro studies. [1] It could increase the percentage of collagen in skin injuries and reduce the severity of inflammation of skin injuries in both diabetic and non-diabetic rats treated with this laser. [1] Using the He-Ne laser in 2 nd degree burn in rats has been associated with a significant increase in mast cells followed by a decreased number of mast cells during inflammatory and remodeling phases of wound healing. [9] Similar mechanisms may be involved in wound healing improvement induced by dental light cure. Low doses of blue light phototherapy destroy hospital- and community-acquired methicillin-resistant S. aureus (HA-MRSA). This antimicrobial effect may be involved in positive effects of blue light on wound repair. [11],[15],[16] A recent report of increased nitric oxide concentration in endothelial cell culture may also be involved in the observed positive effect of blue light on wound healing. [17]

More detailed studies are needed to assess the safety and mechanism of action of blue light therapy for this indication before introducing it for any clinical trial.


  Acknowledgment Top


The authors would like to thank the Skin Disease and Leishmaniasis Research Center, Professor Torabinejad and Science and Research Azad University staff. Special acknowledgment is also due to Dr. Shahriar Adibi, Dr. Ehsan Dehghani Naghvani and Dr. Behnam Abbasian for their kind help and support.

 
  References Top

1.da Silva JP, da Silva MA, Almeida AP, Lombardi Junior I, Matos AP. Laser therapy in the tissue repair process: A literature review. Photomed Laser Surg 2010;28:17-21.  Back to cited text no. 1
    
2.Pinheiro AL. Advances and perspectives on tissue repair and healing.Photomed Laser Surg 2009;27:833-6.  Back to cited text no. 2
    
3.Dazzi F, Horwood NJ. Potential of mesenchymal stem cell therapy. Curr Opin Oncol 2007;19:650-5.  Back to cited text no. 3
    
4.Zhao LL, Davidson JD, Wee SC, Roth SI, Mustoe TA. Effect of hyperbaric oxygen and growth factors on rabbit ear ischemic ulcers. Arch Surg 1994;129:1043-9.  Back to cited text no. 4
    
5.Bouachour G, Cronier P, Gouello JP, Toulemonde JL, Talha A, Alquier P. Hyperbaric oxygen therapy in the management of crush injuries: A randomized double-blind placebo-controlled clinical trial. J Trauma 1996;41:333-9.  Back to cited text no. 5
    
6.Meirelles GC, Santos JN, Chagas PO, Moura AP, Pinheiro AL. A comparative study of the effects of laser photobiomodulation on the healing of third-degree burns: A histological study in rats. Photomed Laser Surg 2008;26:159-66.  Back to cited text no. 6
    
7.Pinheiro AL, Meireles GC, Carvalho CM, de Barros Vieira AL, dos Santos JN, Ramalho LM. Biomodulative effects of polarized light on the healing of cutaneous wounds on nourished and undernourished wistar rats. Photomed Laser Surg 2006;24:616-24.  Back to cited text no. 7
    
8.Whelan HT, Buchmann EV, Dhokalia A, Kane MP, Whelan NT, Wong-Riley MT, et al. Effect of NASA light-emitting diode irradiation on molecular changes for wound healing in diabetic mice. J Clin Laser Med Surg 2003;21:67-74.  Back to cited text no. 8
    
9.Whinfield AL, Aitkenhead I. The light revival: Does phototherapy promote wound healing? A review. Foot (Edinb) 2009;19:117-24.  Back to cited text no. 9
    
10.Vasheghani MM, Bayat M, Rezaei F, Bayat A, Karimipour M. Effect of low-level laser therapy on mast cells in second-degree burns in rats. Photomed Laser Surg 2008;26:1-5.  Back to cited text no. 10
    
11.Enwemeka CS, Williams D, Hollosi S, Yens D, Enwemeka SK. Visible 405 nm SLD light photo-destroys methicillin-resistant Staphylococcus aureus (MRSA) in vitro. Lasers Surg Med 2008;40:734-7.  Back to cited text no. 11
    
12.Woodruff LD, Bounkeo JM, Brannon WM, Dawes KS, Barham CD, Waddell DL, et al. The efficacy of laser therapy in wound repair: A meta-analysis of the literature. Photomed Laser Surg 2004;22:241-7.  Back to cited text no. 12
    
13.Enwemeka CS, Parker JC, Dowdy DS, Harkness EE, Sanford LE, Woodruff LD. The efficacy of low-power lasers in tissue repair and pain control: A meta analysis study. Photomed Laser Surg 2004;22:323-9.  Back to cited text no. 13
    
14.Demidova-Rice TN, Salomatina EV, Yaroslavsky AN, Herman IM, Hamblin MR. Low-level light stimulates excisional wound healing in mice. Lasers Surg 2007;39:706-15.  Back to cited text no. 14
    
15.Enwemeka CS, Williams D, Enwemeka SK, Hollosi S, Yens D. Blue 470-nm light kills methicillin-resistant staphylococcus aureus (MRSA) in vitro.Photomed Laser Surg 2009;27:221-6.  Back to cited text no. 15
    
16.Maclean M, MacGregor SJ, Anderson JG, Woolsey G. High-intensity narrow-spectrum light inactivation and wavelength sensitivity of staphylococcus aureus. FEMS Microbiol Lett 2008;285:227-32.  Back to cited text no. 16
    
17.Ankri R, FriedmanH, Savion N, Kotev-Emeth S, Breitbart H, Lubart R.Visible light induces (NO) formation in sperm and endothelial cells. Laser Surg Med 2010;42:348-52.  Back to cited text no. 17
    


    Figures

  [Figure 1]
 
 
    Tables

  [Table 1]


This article has been cited by
1 Effect of low-level laser therapy on the healing process of donor site in patients with grade 3 burn ulcer after skin graft surgery (a randomized clinical trial)
Reza Vaghardoost,Mahnoush Momeni,Nooshafarin Kazemikhoo,Soheila Mokmeli,Mostafa Dahmardehei,Fereshteh Ansari,Mohammad Ali Nilforoushzadeh,Parisa Sabr joo,Sara Mey Abadi,Soheila Naderi Gharagheshlagh,Saeed Sassani
Lasers in Medical Science. 2018;
[Pubmed] | [DOI]
2 Evaluation of the Effects of Low Level Laser Therapy on the Healing Process After Skin Graft Surgery in Burned Patients (A Randomized Clinical Trial)
Nooshafarin Kazemikhoo,Reza Vaghardoost,Mostafa Dahmardehei,Soheila Mokmeli,Mahnoush Momeni,Mohammad Ali Nilforoushzadeh,Fereshteh Ansari,Mohammad Reza Razagi,Zahra Razagi,Mohammad Amir Amirkhani,Mohammad Reza Masjedi
Journal of Lasers in Medical Sciences. 2018; 9(2): 139
[Pubmed] | [DOI]
3 Effects of low level laser therapy on the prognosis of split-thickness skin graft in type 3 burn of diabetic patients: a case series
Mostafa Dahmardehei,Nooshafarin Kazemikhoo,Reza Vaghardoost,Soheila Mokmeli,Mahnoush Momeni,Mohammad Ali Nilforoushzadeh,Fereshteh Ansari,Amir Amirkhani
Lasers in Medical Science. 2016;
[Pubmed] | [DOI]



 

Top
Previous article  Next article
 
  Search
 
Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
Access Statistics
Email Alert *
Add to My List *
* Registration required (free)

 
  In this article
Abstract
Introduction
Materials and Me...
Results
Discussion
Acknowledgment
References
Article Figures
Article Tables

 Article Access Statistics
    Viewed1372    
    Printed19    
    Emailed0    
    PDF Downloaded227    
    Comments [Add]    
    Cited by others 3    

Recommend this journal